| Title |
Kinetics of Ergothioneine Inhibition of Mushroom Tyrosinase
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, November 2011
|
| DOI | 10.1007/s12010-011-9421-x |
| Pubmed ID | |
| Authors |
Wayne C. Liao, Wen Hong Wu, Pei-Chuan Tsai, Hui-Feng Wang, Yi-Hsin Liu, Chin-Feng Chan |
| Abstract |
The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC(50) value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompetitive tyrosinase inhibitor using a Lineweaver-Burk plot of experimental kinetic data. The IC(50) value for ergothioneine scavenging of 2,2-diphenyl-1-picrylhydrazyl was 6.110 ± 0.305 mg/ml, much higher than the IC(50) for inhibition of tyrosinase activity which indicating ergothioneine on tyrosinase shows a weak correlation to its antioxidative activity. The results demonstrated that ergothioneine has a potent inhibition effect on tyrosinase enzyme activity, resulting from the presence of the sulfur substituted imidazole ring in ergothioneine. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Indonesia | 1 | 5% |
| France | 1 | 5% |
| Unknown | 18 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 20% |
| Student > Ph. D. Student | 4 | 20% |
| Student > Doctoral Student | 3 | 15% |
| Lecturer | 3 | 15% |
| Lecturer > Senior Lecturer | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 4 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 30% |
| Chemistry | 4 | 20% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 15% |
| Computer Science | 1 | 5% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 3 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 November 2023.
All research outputs
#20,232,966
of 24,877,044 outputs
Outputs from Applied Biochemistry and Biotechnology
#1,928
of 2,709 outputs
Outputs of similar age
#122,290
of 147,707 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#21
of 25 outputs
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We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.