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Stem Cell Heterogeneity

Overview of attention for book
Cover of 'Stem Cell Heterogeneity'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 315 Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice
  3. Altmetric Badge
    Chapter 319 Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro
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    Chapter 320 Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro
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    Chapter 321 Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies
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    Chapter 322 An Effective and Reliable Xeno-free Cryopreservation Protocol for Single Human Pluripotent Stem Cells
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    Chapter 323 Induction of a Tumor-Metastasis-Receptive Microenvironment as an Unwanted Side Effect After Radio/Chemotherapy and In Vitro and In Vivo Assays to Study this Phenomenon
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    Chapter 324 Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing
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    Chapter 325 Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity
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    Chapter 326 Isolation and Characterization of Cancer Stem Cells of the Non-Small-Cell Lung Cancer (A549) Cell Line
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    Chapter 327 Aerosol-Based Cell Therapy for Treatment of Lung Diseases
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    Chapter 327 Erratum to: Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method
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    Chapter 328 Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro
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    Chapter 329 In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry
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    Chapter 342 Isolation and Propagation of Glioma Stem Cells from Acutely Resected Tumors
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    Chapter 343 Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter
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    Chapter 344 Automated Cell-Based Quantitation of 8-OHdG Damage
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    Chapter 345 Heterogeneity of Stem Cells: A Brief Overview
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    Chapter 346 Agent-Based Modeling of Cancer Stem Cell Driven Solid Tumor Growth.
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    Chapter 347 Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells.
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    Chapter 348 Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method
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    Chapter 349 Visualizing the Functional Heterogeneity of Muscle Stem Cells
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    Chapter 350 CoCl2 Administration to Vascular MSC Cultures as an In Vitro Hypoxic System to Study Stem Cell Survival and Angiogenesis
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    Chapter 356 Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells
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    Chapter 357 Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs)
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    Chapter 358 Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells.
  27. Altmetric Badge
    Chapter 360 Reporter Systems to Study Cancer Stem Cells
  28. Altmetric Badge
    Chapter 361 Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells
  29. Altmetric Badge
    Chapter 362 Growth Factor-Free Pre-vascularization of Cell Sheets for Tissue Engineering
Attention for Chapter 358: Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells.
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Chapter title
Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells.
Chapter number 358
Book title
Stem Cell Heterogeneity
Published in
Methods in molecular biology, May 2016
DOI 10.1007/7651_2016_358
Pubmed ID
27138010
Book ISBNs
978-1-4939-6549-6, 978-1-4939-6550-2
Authors

Krzysztof Szade, Monika Zukowska, Alicja Jozkowicz, Jozef Dulak, Szade, Krzysztof, Zukowska, Monika, Jozkowicz, Alicja, Dulak, Jozef

Abstract

The metabolism of quiescent adult stem cells differs from the metabolism of differentiated cells. The metabolic processes are tightly regulated and their alterations disturb function of stem cells. One of the indicators of metabolic status of cells is the ATP level. While the method of measuring the ATP levels has been known for many years, estimating ATP levels in small population of defined stem cells isolated directly from the tissue has remained challenging. Here, we show our method of measuring the ATP levels in hematopoietic stem cells sorted from murine bone marrow. We used magnetic sorting as well as cell sorter and adopted the commonly used bioluminescence-based detection kits in described protocol. Our strategy allows to measure ATP levels in 1000 highly purified HSC.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 20%
Other 1 10%
Student > Bachelor 1 10%
Professor 1 10%
Student > Master 1 10%
Other 2 20%
Unknown 2 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 20%
Agricultural and Biological Sciences 2 20%
Nursing and Health Professions 1 10%
Medicine and Dentistry 1 10%
Chemistry 1 10%
Other 1 10%
Unknown 2 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 May 2016.
All research outputs
#18,456,836
of 22,869,263 outputs
Outputs from Methods in molecular biology
#7,924
of 13,129 outputs
Outputs of similar age
#218,711
of 298,762 outputs
Outputs of similar age from Methods in molecular biology
#18
of 27 outputs
Altmetric has tracked 22,869,263 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,129 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.

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