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A portable approach for the surveillance of dengue virus-infected mosquitoes

Overview of attention for article published in Journal of Virological Methods, April 2012
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Title
A portable approach for the surveillance of dengue virus-infected mosquitoes
Published in
Journal of Virological Methods, April 2012
DOI 10.1016/j.jviromet.2012.03.033
Pubmed ID
Authors

David A. Muller, Francesca D. Frentiu, Alejandra Rojas, Luciano A. Moreira, Scott L. O’Neill, Paul R. Young

Abstract

Dengue virus is the most significant human viral pathogen spread by the bite of an infected mosquito. With no vaccine or antiviral therapy currently available, disease prevention relies largely on surveillance and mosquito control. Preventing the onset of dengue outbreaks and effective vector management would be considerably enhanced through surveillance of dengue virus prevalence in natural mosquito populations. However, current approaches to the identification of virus in field-caught mosquitoes require relatively slow and labor intensive techniques such as virus isolation or RT-PCR involving specialized facilities and personnel. A rapid and portable method for detecting dengue virus-infected mosquitoes is described. Using a hand held battery operated homogenizer and a dengue diagnostic rapid strip the viral protein NS1 was detected as a marker of dengue virus infection. This method could be performed in less than 30 min in the field, requiring no downstream processing, and is able to detect a single infected mosquito in a pool of at least 50 uninfected mosquitoes. The method described in this study allows rapid, real-time monitoring of dengue virus presence in mosquito populations and could be a useful addition to effective monitoring and vector control responses.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 <1%
French Polynesia 1 <1%
Indonesia 1 <1%
United Kingdom 1 <1%
Canada 1 <1%
United States 1 <1%
Unknown 96 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 35 34%
Student > Ph. D. Student 14 14%
Student > Bachelor 9 9%
Student > Master 8 8%
Other 5 5%
Other 20 20%
Unknown 11 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 51 50%
Biochemistry, Genetics and Molecular Biology 9 9%
Medicine and Dentistry 9 9%
Immunology and Microbiology 3 3%
Chemistry 3 3%
Other 11 11%
Unknown 16 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 May 2012.
All research outputs
#14,536,995
of 25,374,917 outputs
Outputs from Journal of Virological Methods
#2,318
of 3,282 outputs
Outputs of similar age
#96,255
of 173,343 outputs
Outputs of similar age from Journal of Virological Methods
#17
of 29 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,282 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.5. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 173,343 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.