Title |
SOCS3 Is a Critical Physiological Negative Regulator of G-CSF Signaling and Emergency Granulopoiesis
|
---|---|
Published in |
Immunity, February 2004
|
DOI | 10.1016/s1074-7613(04)00022-6 |
Pubmed ID | |
Authors |
Ben A Croker, Donald Metcalf, Lorraine Robb, Wei Wei, Sandra Mifsud, Ladina DiRago, Leonie A Cluse, Kate D Sutherland, Lynne Hartley, Emily Williams, Jian-Guo Zhang, Douglas J Hilton, Nicos A Nicola, Warren S Alexander, Andrew W Roberts |
Abstract |
To determine the importance of suppressor of cytokine signaling-3 (SOCS3) in the regulation of hematopoietic growth factor signaling generally, and of G-CSF-induced cellular responses specifically, we created mice in which the Socs3 gene was deleted in all hematopoietic cells. Although normal until young adulthood, these mice then developed neutrophilia and a spectrum of inflammatory pathologies. When stimulated with G-CSF in vitro, SOCS3-deficient cells of the neutrophilic granulocyte lineage exhibited prolonged STAT3 activation and enhanced cellular responses to G-CSF, including an increase in cloning frequency, survival, and proliferative capacity. Consistent with the in vitro findings, mutant mice injected with G-CSF displayed enhanced neutrophilia, progenitor cell mobilization, and splenomegaly, but unexpectedly also developed inflammatory neutrophil infiltration into multiple tissues and consequent hind-leg paresis. We conclude that SOCS3 is a key negative regulator of G-CSF signaling in myeloid cells and that this is of particular significance during G-CSF-driven emergency granulopoiesis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 2% |
China | 1 | <1% |
Australia | 1 | <1% |
Unknown | 163 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 28 | 17% |
Student > Ph. D. Student | 27 | 16% |
Student > Master | 10 | 6% |
Student > Bachelor | 10 | 6% |
Professor | 7 | 4% |
Other | 13 | 8% |
Unknown | 73 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 30 | 18% |
Biochemistry, Genetics and Molecular Biology | 24 | 14% |
Medicine and Dentistry | 15 | 9% |
Immunology and Microbiology | 7 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
Other | 16 | 10% |
Unknown | 73 | 43% |