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X Demographics
Mendeley readers
| Title |
A Chemical Genetics Approach Reveals H,K-ATPase-Mediated Membrane Voltage Is Required for Planarian Head Regeneration
|
|---|---|
| Published in |
Cell Chemical Biology, January 2011
|
| DOI | 10.1016/j.chembiol.2010.11.012 |
| Pubmed ID | |
| Authors |
Wendy S. Beane, Junji Morokuma, Dany S. Adams, Michael Levin |
| Abstract |
Biophysical signaling is required for both embryonic polarity and regenerative outgrowth. Exploiting endogenous ion transport for regenerative therapies will require direct regulation of membrane voltage. Here, we develop a pharmacological method to target ion transporters, uncovering a role for membrane voltage as a key regulator of anterior polarity in regenerating planaria. Utilizing the highly specific inhibitor, SCH-28080, our data reveal that H(+),K(+)-ATPase-mediated membrane depolarization is essential for anterior gene expression and brain induction. H(+),K(+)-ATPase-independent manipulation of membrane potential with ivermectin confirms that depolarization drives head formation, even at posterior-facing wounds. Using this chemical genetics approach, we demonstrate that membrane voltage controls head-versus-tail identity during planarian regeneration. Our data suggest well-characterized drugs (already approved for human use) might be exploited to control adult stem cell-driven pattern formation during the regeneration of complex structures. |
X Demographics
The data shown below were collected from the profiles of 16 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 25% |
| Germany | 1 | 6% |
| France | 1 | 6% |
| Netherlands | 1 | 6% |
| Japan | 1 | 6% |
| Austria | 1 | 6% |
| Unknown | 7 | 44% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 11 | 69% |
| Scientists | 4 | 25% |
| Practitioners (doctors, other healthcare professionals) | 1 | 6% |
Mendeley readers
The data shown below were compiled from readership statistics for 153 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 3% |
| United Kingdom | 1 | <1% |
| Germany | 1 | <1% |
| Unknown | 147 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 35 | 23% |
| Researcher | 26 | 17% |
| Student > Bachelor | 24 | 16% |
| Student > Master | 14 | 9% |
| Professor > Associate Professor | 11 | 7% |
| Other | 22 | 14% |
| Unknown | 21 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 63 | 41% |
| Biochemistry, Genetics and Molecular Biology | 33 | 22% |
| Medicine and Dentistry | 11 | 7% |
| Neuroscience | 6 | 4% |
| Engineering | 3 | 2% |
| Other | 11 | 7% |
| Unknown | 26 | 17% |