| Title |
A large geometric distortion in the first photointermediate of rhodopsin, determined by double-quantum solid-state NMR
|
|---|---|
| Published in |
Journal of Biomolecular NMR, May 2012
|
| DOI | 10.1007/s10858-012-9635-4 |
| Pubmed ID | |
| Authors |
Maria Concistrè, Ole G. Johannessen, Neville McLean, Petra H. M. Bovee-Geurts, Richard C. D. Brown, Willem J. DeGrip, Malcolm H. Levitt |
| Abstract |
Double-quantum magic-angle-spinning NMR experiments were performed on 11,12-(13)C(2)-retinylidene-rhodopsin under illumination at low temperature, in order to characterize torsional angle changes at the C11-C12 photoisomerization site. The sample was illuminated in the NMR rotor at low temperature (~120 K) in order to trap the primary photointermediate, bathorhodopsin. The NMR data are consistent with a strong torsional twist of the HCCH moiety at the isomerization site. Although the HCCH torsional twist was determined to be at least 40°, it was not possible to quantify it more closely. The presence of a strong twist is in agreement with previous Raman observations. The energetic implications of this geometric distortion are discussed. |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 4% |
| France | 1 | 4% |
| Unknown | 23 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 28% |
| Student > Bachelor | 3 | 12% |
| Student > Ph. D. Student | 3 | 12% |
| Student > Master | 3 | 12% |
| Professor | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 4 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 14 | 56% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Agricultural and Biological Sciences | 1 | 4% |
| Neuroscience | 1 | 4% |
| Materials Science | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 6 | 24% |