Title |
Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources
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Published in |
International Journal of Systematic and Evolutionary Microbiology, April 2015
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DOI | 10.1099/ijs.0.000251 |
Pubmed ID | |
Authors |
Birgit De Smet, Mark Mayo, Charlotte Peeters, James E A Zlosnik, Theodore Spilker, Trevor J Hird, John J LiPuma, Timothy J Kidd, Mirjam Kaestli, Jennifer L Ginther, David M Wagner, Paul Keim, Scott C Bell, Jan A Jacobs, Bart J Currie, Peter Vandamme |
Abstract |
Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the etiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4% and 1.9%, respectively) and towards established Bcc species (4.0% and 3.9%, respectively) demonstrated that both taxa represented novel Bcc species. All twelve isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis, and biochemical profiling. recA gene sequence analysis revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of both taxa to the Burkholderia cepacia complex. Bcc B strains can be differentiated from most other Bcc by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156T (CCUG 65686T) and Burkholderia territorii sp. nov. with type strain LMG 28158T (CCUG 65687T), are proposed for Bcc B and Bcc L bacteria, respectively. |
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