| Title |
Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, April 2015
|
| DOI | 10.1007/s00253-015-6548-7 |
| Pubmed ID | |
| Authors |
Margo Diricks, Frederik De Bruyn, Paul Van Daele, Maarten Walmagh, Tom Desmet |
| Abstract |
Sucrose synthase (SuSy) catalyzes the reversible conversion of sucrose and a nucleoside diphosphate into fructose and nucleotide (NDP)-glucose. To date, only SuSy's from plants and cyanobacteria, both photosynthetic organisms, have been characterized. Here, four prokaryotic SuSy enzymes from the nonphotosynthetic organisms Nitrosomonas Europaea (SuSyNe), Acidithiobacillus caldus (SuSyAc), Denitrovibrio acetiphilus (SusyDa), and Melioribacter roseus (SuSyMr) were recombinantly expressed in Escherichia coli and thoroughly characterized. The purified enzymes were found to display high-temperature optima (up to 80 °C), high activities (up to 125 U/mg), and high thermostability (up to 15 min at 60 °C). Furthermore, SuSyAc, SuSyNe, and SuSyDa showed a clear preference for ADP as nucleotide, as opposed to plant SuSy's which prefer UDP. A structural and mutational analysis was performed to elucidate the difference in NDP preference between eukaryotic and prokaryotic SuSy's. Finally, the physiological relevance of this enzyme specificity is discussed in the context of metabolic pathways and genomic organization. |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 1% |
| Unknown | 66 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 17 | 25% |
| Researcher | 13 | 19% |
| Student > Ph. D. Student | 8 | 12% |
| Student > Bachelor | 7 | 10% |
| Other | 3 | 4% |
| Other | 7 | 10% |
| Unknown | 12 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 26 | 39% |
| Agricultural and Biological Sciences | 16 | 24% |
| Chemistry | 5 | 7% |
| Business, Management and Accounting | 1 | 1% |
| Environmental Science | 1 | 1% |
| Other | 5 | 7% |
| Unknown | 13 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2024.
All research outputs
#3,625,854
of 24,535,155 outputs
Outputs from Applied Microbiology and Biotechnology
#552
of 8,137 outputs
Outputs of similar age
#46,270
of 269,519 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#12
of 120 outputs
Altmetric has tracked 24,535,155 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,137 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 88% of its peers.
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We're also able to compare this research output to 120 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.