| Title |
Characterization of a simplified ice-free cryopreservation method for heart valves
|
|---|---|
| Published in |
Cell and Tissue Banking, June 2012
|
| DOI | 10.1007/s10561-012-9319-z |
| Pubmed ID | |
| Authors |
Agnes J. T. Huber, Timo Aberle, Martina Schleicher, Hans-Peter Wendel, Kelvin G. M. Brockbank |
| Abstract |
The aim of the present study was to characterize the hemocompatibility of ice-free cryopreserved heart valves in anticipation of future human trials. Porcine pulmonary heart valves were infiltrated with either an 83 % cryoprotectant solution followed by rapid cooling and storage at --80 °C or with 10 % DMSO and control rate freezing to --80 °C and storage in vapor phase nitrogen as conventional frozen controls. Cryopreserved leaflets were compared with fresh, decellularized and glutaraldehyde-fixed control valve leaflets using a battery of coagulation protein assays after exposure to human blood. Von Willebrand Factor staining indicated that most of the endothelium was lost during valve processing prior to cryopreservation. Hemocompatibility, employing thrombin/antithrombin-III-complex, polymorphonuclear neutrophil-elastase, beta-thromboglobulin and terminal complement complex SC5b-9, was preserved compared with both fresh and frozen leaflets. Hemocompatibility differences were observed for cryopreserved leaflets versus both decellularized and glutaraldehyde fixed controls. In conclusion, the hemocompatibility results support the use of ice-free cryopreservation as a simplified preservation method because no statistically significant differences in hemocompatibility were observed between the two cryopreservation methods and fresh untreated controls. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 25% |
| Student > Master | 3 | 19% |
| Student > Bachelor | 2 | 13% |
| Other | 2 | 13% |
| Lecturer | 1 | 6% |
| Other | 3 | 19% |
| Unknown | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 38% |
| Biochemistry, Genetics and Molecular Biology | 2 | 13% |
| Engineering | 2 | 13% |
| Computer Science | 1 | 6% |
| Veterinary Science and Veterinary Medicine | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 2 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 June 2012.
All research outputs
#14,147,011
of 22,669,724 outputs
Outputs from Cell and Tissue Banking
#142
of 286 outputs
Outputs of similar age
#96,402
of 164,469 outputs
Outputs of similar age from Cell and Tissue Banking
#3
of 4 outputs
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