Title |
CIS is a potent checkpoint in NK cell–mediated tumor immunity
|
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Published in |
Nature Immunology, May 2016
|
DOI | 10.1038/ni.3470 |
Pubmed ID | |
Authors |
Rebecca B Delconte, Tatiana B Kolesnik, Laura F Dagley, Jai Rautela, Wei Shi, Eva M Putz, Kimberley Stannard, Jian-Guo Zhang, Charis Teh, Matt Firth, Takashi Ushiki, Christopher E Andoniou, Mariapia A Degli-Esposti, Phillip P Sharp, Caroline E Sanvitale, Giuseppe Infusini, Nicholas P D Liau, Edmond M Linossi, Christopher J Burns, Sebastian Carotta, Daniel H D Gray, Cyril Seillet, Dana S Hutchinson, Gabrielle T Belz, Andrew I Webb, Warren S Alexander, Shawn S Li, Alex N Bullock, Jeffery J Babon, Mark J Smyth, Sandra E Nicholson, Nicholas D Huntington |
Abstract |
The detection of aberrant cells by natural killer (NK) cells is controlled by the integration of signals from activating and inhibitory ligands and from cytokines such as IL-15. We identified cytokine-inducible SH2-containing protein (CIS, encoded by Cish) as a critical negative regulator of IL-15 signaling in NK cells. Cish was rapidly induced in response to IL-15, and deletion of Cish rendered NK cells hypersensitive to IL-15, as evidenced by enhanced proliferation, survival, IFN-γ production and cytotoxicity toward tumors. This was associated with increased JAK-STAT signaling in NK cells in which Cish was deleted. Correspondingly, CIS interacted with the tyrosine kinase JAK1, inhibiting its enzymatic activity and targeting JAK for proteasomal degradation. Cish(-/-) mice were resistant to melanoma, prostate and breast cancer metastasis in vivo, and this was intrinsic to NK cell activity. Our data uncover a potent intracellular checkpoint in NK cell-mediated tumor immunity and suggest possibilities for new cancer immunotherapies directed at blocking CIS function. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 26 | 26% |
United Kingdom | 5 | 5% |
Australia | 4 | 4% |
Canada | 4 | 4% |
Mexico | 3 | 3% |
Paraguay | 2 | 2% |
Venezuela, Bolivarian Republic of | 2 | 2% |
Netherlands | 1 | 1% |
Ireland | 1 | 1% |
Other | 11 | 11% |
Unknown | 40 | 40% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 73 | 74% |
Scientists | 16 | 16% |
Practitioners (doctors, other healthcare professionals) | 7 | 7% |
Science communicators (journalists, bloggers, editors) | 3 | 3% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Denmark | 2 | <1% |
United States | 2 | <1% |
Australia | 1 | <1% |
Japan | 1 | <1% |
Germany | 1 | <1% |
Unknown | 332 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 91 | 27% |
Student > Ph. D. Student | 60 | 18% |
Student > Master | 35 | 10% |
Student > Bachelor | 32 | 9% |
Other | 22 | 6% |
Other | 46 | 14% |
Unknown | 53 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 81 | 24% |
Immunology and Microbiology | 79 | 23% |
Biochemistry, Genetics and Molecular Biology | 59 | 17% |
Medicine and Dentistry | 29 | 9% |
Chemistry | 9 | 3% |
Other | 25 | 7% |
Unknown | 57 | 17% |