| Title |
High-level expression and characterization of a thermophilic β-mannanase from Aspergillus niger in Pichia pastoris
|
|---|---|
| Published in |
Biotechnology Techniques, May 2015
|
| DOI | 10.1007/s10529-015-1848-7 |
| Pubmed ID | |
| Authors |
Shi Yu, Zhezhe Li, Yaping Wang, Wanping Chen, Lin Fu, Wei Tang, Chen Chen, Yunyun Liu, Xue Zhang, Lixin Ma |
| Abstract |
A novel, high-level expression, thermostable mannan endo-1,4-beta-mannosidase is urgently needed for industrial applications. The mannan endo-1,4-β-mannosidase gene (MAN) from Aspergillus niger CBS 513.88 was optimized based on the codon usage bias in Pichia pastoris and synthesized by overlapping PCR to produce MAN-P. It was expressed in P. pastoris GS115 from a constitutive expression vector pHBM-905 M. MAN-P reached 594 mg/l in shake-flasks after 192 h induction. On production in a 5 l fermenter, the yield of MAN-P reached ~3.5 mg/ml and the enzyme activity was 1612 U/ml. The enzyme exhibited a maximum activity of 3049 U/ml at 80 °C and retained 60 % enzyme activity at 80 °C for 2 h. The pH optimum was 4.5 and the enzyme was stable over the pH range 1.5-11. The thermostability of MAN-P is higher than other known fungal mannanases and the expression and thermophilic properties make MAN-P useful for industrial applications. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 24% |
| Student > Ph. D. Student | 5 | 20% |
| Student > Bachelor | 3 | 12% |
| Researcher | 3 | 12% |
| Student > Doctoral Student | 2 | 8% |
| Other | 1 | 4% |
| Unknown | 5 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 28% |
| Agricultural and Biological Sciences | 5 | 20% |
| Immunology and Microbiology | 2 | 8% |
| Engineering | 2 | 8% |
| Medicine and Dentistry | 2 | 8% |
| Other | 1 | 4% |
| Unknown | 6 | 24% |
Attention Score in Context
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#22,758,309
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Outputs from Biotechnology Techniques
#2,492
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#238,997
of 279,148 outputs
Outputs of similar age from Biotechnology Techniques
#22
of 30 outputs
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