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Mendeley readers
Attention Score in Context
| Title |
Anisotropy resolved multidimensional emission spectroscopy (ARMES): A new tool for protein analysis
|
|---|---|
| Published in |
Analytica Chimica Acta, July 2015
|
| DOI | 10.1016/j.aca.2015.06.011 |
| Pubmed ID | |
| Authors |
Radu Constantin Groza, Boyan Li, Alan G. Ryder |
| Abstract |
Structural analysis of proteins using the emission of intrinsic fluorophores is complicated by spectral overlap. Anisotropy resolved multidimensional emission spectroscopy (ARMES) overcame the overlap problem by the use of anisotropy, with chemometric analysis, to better resolve emission from different fluorophores. Total synchronous fluorescence scan (TSFS) provided information about all the fluorophores that contributed to emission while anisotropy provided information about the environment of each fluorophore. Here the utility of ARMES was demonstrated via study of the chemical and thermal denaturation of human serum albumin (HSA). Multivariate curve resolution (MCR) analysis of the constituent polarized emission ARMES data resolved contributions from four emitters: fluorescence from tryptophan (Trp), solvent exposed tyrosine (Tyr), Tyr in a hydrophobic environment, and room temperature phosphorescence (RTP) from Trp. The MCR scores, anisotropy, and literature validated these assignments and showed all the expected transitions during HSA unfolding. This new methodology for comprehensive intrinsic fluorescence analysis of proteins is applicable to any protein containing multiple fluorophores. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 1 | 4% |
| Unknown | 25 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 31% |
| Student > Master | 5 | 19% |
| Researcher | 4 | 15% |
| Student > Doctoral Student | 2 | 8% |
| Professor | 2 | 8% |
| Other | 4 | 15% |
| Unknown | 1 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 10 | 38% |
| Agricultural and Biological Sciences | 5 | 19% |
| Physics and Astronomy | 3 | 12% |
| Engineering | 2 | 8% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 1 | 4% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 May 2016.
All research outputs
#22,759,802
of 25,374,917 outputs
Outputs from Analytica Chimica Acta
#6,707
of 7,521 outputs
Outputs of similar age
#235,472
of 276,133 outputs
Outputs of similar age from Analytica Chimica Acta
#39
of 49 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,521 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 276,133 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.