| Title |
Hydrodynamic Radii of Ranibizumab, Aflibercept and Bevacizumab Measured by Time-Resolved Phosphorescence Anisotropy
|
|---|---|
| Published in |
Pharmaceutical Research, May 2016
|
| DOI | 10.1007/s11095-016-1940-2 |
| Pubmed ID | |
| Authors |
Liisa M. Hirvonen, Gilbert O. Fruhwirth, Nishanthan Srikantha, Matthew J. Barber, James E. Neffendorf, Klaus Suhling, Timothy L. Jackson |
| Abstract |
To measure the hydrodynamic radii of intravitreal anti-VEGF drugs ranibizumab, aflibercept and bevacizumab with μs time-resolved phosphorescence anisotropy. Ruthenium-based dye Ru(bpy)2(mcbpy - O - Su - ester)(PF6)2, whose lifetime of several hundred nanoseconds is comparable to the rotational correlation time of these drugs in buffer, was used as a label. The hydrodynamic radii were calculated from the rotational correlation times of the Ru(bpy)2(mcbpy - O - Su - ester)(PF6)2-labelled drugs obtained with time-resolved phosphorescence anisotropy measurements in buffer/glycerol solutions of varying viscosity. The measured radii of 2.76±0.04 nm for ranibizumab, 3.70±0.03 nm for aflibercept and 4.58±0.01 nm for bevacizumab agree with calculations based on molecular weight and other experimental measurements. Time-resolved phosphorescence anisotropy is a relatively simple and straightforward method that allows experimental measurement of the hydrodynamic radius of individual proteins, and is superior to theoretical calculations which cannot give the required accuracy for a particular protein. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| Unknown | 59 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 28% |
| Researcher | 13 | 22% |
| Student > Master | 3 | 5% |
| Student > Bachelor | 2 | 3% |
| Student > Doctoral Student | 2 | 3% |
| Other | 5 | 8% |
| Unknown | 18 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 8 | 13% |
| Biochemistry, Genetics and Molecular Biology | 7 | 12% |
| Chemistry | 7 | 12% |
| Agricultural and Biological Sciences | 5 | 8% |
| Chemical Engineering | 3 | 5% |
| Other | 11 | 18% |
| Unknown | 19 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2023.
All research outputs
#7,868,283
of 24,387,992 outputs
Outputs from Pharmaceutical Research
#1,056
of 2,944 outputs
Outputs of similar age
#118,998
of 341,940 outputs
Outputs of similar age from Pharmaceutical Research
#27
of 52 outputs
Altmetric has tracked 24,387,992 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 2,944 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one has gotten more attention than average, scoring higher than 63% of its peers.
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