You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Proteomic changes occurring in the malaria mosquitoes Anopheles gambiae and Anopheles stephensi during aging
|
|---|---|
| Published in |
Journal of Proteomics, June 2015
|
| DOI | 10.1016/j.jprot.2015.06.008 |
| Pubmed ID | |
| Authors |
Maggy T. Sikulu, James Monkman, Keyur A. Dave, Marcus L. Hastie, Patricia E. Dale, Roger L. Kitching, Gerry F. Killeen, Brian H. Kay, Jeffery J. Gorman, Leon E. Hugo |
| Abstract |
The age of mosquitoes is a crucial determinant of their ability to transmit pathogens and resistance to insecticides. We investigated changes to the abundance of proteins found in heads and thoraces of the malaria mosquitoes Anopheles gambiae and Anopheles stephensi as they aged. Protein expression changes were assessed using two-dimensional difference gel electrophoresis and the identity of differentially expressed proteins was determined by using either matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry or capillary high-pressure liquid chromatography coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometer. Protein biomarkers were validated by semi quantitative Western blot analysis. Nineteen and nine age dependent protein spots were identified for An. stephensi and An. gambiae, respectively. Among the proteins down-regulated with age were homologs of ADF/Cofilin, Cytochome c1, Heat shock protein-70 and Eukaryotic translation initiation factor 5A (eIF5a). Proteins up-regulated with age included Probable methylmalonate-semialdehyde dehydrogenase, Voltage-dependent anion-selective channel and Fructose bisphosphate aldolase. Semi quantitative Western blot analysis confirmed expression patterns observed by 2-D DIGE for eIF5a and ADF/Cofilin. Further work is recommended to determine whether these biomarkers are robust to infection, blood feeding and insecticide resistance. Robust biomarkers could then be incorporated into rapid diagnostic assays for ecological and epidemiological studies. In this study, we have identified several proteins with characteristic changes in abundance in both An. gambiae and An. stephensi during their aging process. These changes may highlight underlying mechanisms beneath the relationship between mosquito age and factors affecting Plasmodium transmission and mosquito control. The similarity of changes in protein abundance between these species and the primary dengue vector Aedes aegypti, has revealed conserved patterns of aging-specific protein regulation. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 92 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 2% |
| Unknown | 90 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 16% |
| Student > Bachelor | 12 | 13% |
| Student > Ph. D. Student | 11 | 12% |
| Student > Master | 11 | 12% |
| Other | 7 | 8% |
| Other | 10 | 11% |
| Unknown | 26 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 20 | 22% |
| Biochemistry, Genetics and Molecular Biology | 13 | 14% |
| Medicine and Dentistry | 13 | 14% |
| Nursing and Health Professions | 6 | 7% |
| Veterinary Science and Veterinary Medicine | 1 | 1% |
| Other | 9 | 10% |
| Unknown | 30 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2016.
All research outputs
#22,756,649
of 25,371,288 outputs
Outputs from Journal of Proteomics
#3,066
of 3,461 outputs
Outputs of similar age
#237,256
of 278,553 outputs
Outputs of similar age from Journal of Proteomics
#66
of 77 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,461 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,553 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.