| Title |
Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples
|
|---|---|
| Published in |
World Journal of Microbiology and Biotechnology, July 2012
|
| DOI | 10.1007/s11274-012-1123-1 |
| Pubmed ID | |
| Authors |
Yolanda Paola Maturano, María Cristina Nally, María Eugenia Toro, Lucía Inés Castellanos de Figueroa, Mariana Combina, Fabio Vazquez |
| Abstract |
Killer yeasts are frequently used to combat and prevent contamination by wild-type yeasts during wine production and they can even dominate the wine fermentation. Stuck and sluggish fermentations can be caused by an unbalanced ratio of killer to sensitive yeasts in the bioreactor, and therefore it is important to determine the proportion of both populations. The aim of this study was to provide a simple tool to monitor killer yeast populations during controlled mixed microvinifications of killer and sensitive Saccharomyces cerevisiae. Samples were periodically extracted during vinification, seeded on Petri dishes and incubated at 25 and 37 °C; the latter temperature was assayed for possible inactivation of killer toxin production. Colonies developed under the described conditions were randomly transferred to killer phenotype detection medium. Significant differences in the killer/sensitive ratio were observed between both incubation temperatures in all microvinifications. These results suggest that 37 °C seems a better option to determine the biomass of sensitive yeasts, in order to avoid underestimation of sensitive cells in the presence of killer yeasts during fermentations. Incubation at a toxin-inhibiting temperature clearly showed the real ratio of killer to sensitive cells in fermentation systems. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 3% |
| United States | 1 | 3% |
| Canada | 1 | 3% |
| Unknown | 36 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 26% |
| Student > Ph. D. Student | 6 | 15% |
| Student > Bachelor | 4 | 10% |
| Student > Doctoral Student | 4 | 10% |
| Student > Master | 4 | 10% |
| Other | 6 | 15% |
| Unknown | 5 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 46% |
| Biochemistry, Genetics and Molecular Biology | 7 | 18% |
| Immunology and Microbiology | 3 | 8% |
| Nursing and Health Professions | 1 | 3% |
| Business, Management and Accounting | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 7 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 July 2012.
All research outputs
#19,440,618
of 23,911,072 outputs
Outputs from World Journal of Microbiology and Biotechnology
#1,233
of 1,757 outputs
Outputs of similar age
#129,243
of 166,636 outputs
Outputs of similar age from World Journal of Microbiology and Biotechnology
#10
of 15 outputs
Altmetric has tracked 23,911,072 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,757 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 166,636 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.