| Title |
Effects of low-level laser therapy on ROS homeostasis and expression of IGF-1 and TGF-β1 in skeletal muscle during the repair process
|
|---|---|
| Published in |
Lasers in Medical Science, June 2012
|
| DOI | 10.1007/s10103-012-1133-0 |
| Pubmed ID | |
| Authors |
Li Luo, Zhongwen Sun, Lin Zhang, Xiaoning Li, Yu Dong, Timon Cheng-Yi Liu |
| Abstract |
The aim of the present study was to determine the effects of low-level laser therapy (LLLT) on the homeostasis of reactive oxygen species (ROS) and expression of IGF-1 and TGF-β1 in the gastrocnemius muscles of rats following contusion. Muscle regeneration involves cell proliferation, migration, and differentiation and is regulated by growth factors. A growing body of evidence suggests that LLLT promotes skeletal muscle regeneration and accelerates tissue repair. Adult male Sprague-Dawley rats (n=96) were randomly divided into three groups: control group (no lesion, untreated, n=6), contusion group (n=48), and contusion-plus-LLLT group (n=42). Gallium aluminum arsenide (GaAlAs) laser irradiation (635 nm; beam spot, 0.4 cm(2); output power, 7 mW; power density, 17.5 mW/cm(2); 20 min) was administered to the gastrocnemius contusion for 20 min daily for 10 days. Muscle remodeling was evaluated at 0 h and 1, 2, 3, 7, 14, 21, and 28 days after injury. Hematoxylin and eosin and Van Gieson staining were used to evaluate regeneration and fibrosis; muscle superoxide dismutase (SOD) and malondialdehyde (MDA) were detected via biochemical methods; expression of transforming growth factor beta-1 (TGF-β1) and insulin-like growth factor-1 (IGF-1) were investigated via immunohistochemistry. The results showed that LLLT markedly promoted the regeneration of muscle and reduced scar formation. LLLT also significantly enhanced muscle SOD activity and significantly decreased muscle MDA levels 1, 2, and 3 days after injury. LLLT increased the expression of IGF-1 2, 3, and 7 days after injury and decreased the expression of IGF-1 21 and 28 days after injury. LLLT decreased the expression of TGF-β1 3 and 28 days after injury but increased expression at 7 and 14 days after injury. Our study showed that LLLT could modulate the homeostasis of ROS and of the growth factors IGF-1 and TGF-β1, which are known to play important roles in the repair process. This may constitute a new preventive approach to muscular fibrosis. |
Mendeley readers
The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | <1% |
| Mexico | 1 | <1% |
| Czechia | 1 | <1% |
| South Africa | 1 | <1% |
| Unknown | 98 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 19 | 19% |
| Student > Master | 18 | 18% |
| Student > Bachelor | 12 | 12% |
| Student > Ph. D. Student | 12 | 12% |
| Professor | 7 | 7% |
| Other | 16 | 16% |
| Unknown | 18 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 25 | 25% |
| Agricultural and Biological Sciences | 17 | 17% |
| Biochemistry, Genetics and Molecular Biology | 12 | 12% |
| Nursing and Health Professions | 6 | 6% |
| Sports and Recreations | 4 | 4% |
| Other | 13 | 13% |
| Unknown | 25 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2020.
All research outputs
#3,991,705
of 22,671,366 outputs
Outputs from Lasers in Medical Science
#80
of 1,303 outputs
Outputs of similar age
#27,495
of 163,879 outputs
Outputs of similar age from Lasers in Medical Science
#2
of 20 outputs
Altmetric has tracked 22,671,366 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,303 research outputs from this source. They receive a mean Attention Score of 3.0. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 163,879 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.