| Title |
Design of a CRISPR-Cas system to increase resistance of Bacillus subtilis to bacteriophage SPP1
|
|---|---|
| Published in |
Journal of Industrial Microbiology & Biotechnology, August 2016
|
| DOI | 10.1007/s10295-016-1784-0 |
| Pubmed ID | |
| Authors |
Lina Jakutyte-Giraitiene, Giedrius Gasiunas |
| Abstract |
Clustered regularly interspaced short palindromic repeats (CRISPR) together with CRISPR-associated (cas) genes form an adaptive prokaryotic immune system which provides acquired resistance against viruses and plasmids. Bacillus subtilis presently is the best-characterized laboratory model for Gram-positive bacteria and also widely used for industrial production of enzymes, vitamins and antibiotics. In this study, we show that type II-A CRISPR-Cas system from Streptococcus thermophilus can be transferred into B. subtilis and provides heterologous protection against phage infection. We engineered a heterologous host by cloning S. thermophilus Cas9 and a spacer targeting bacteriophage SPP1 into the chromosome of B. subtilis, which does not harbor its own CRISPR-Cas systems. We found that the heterologous CRISPR-Cas system is functionally active in B. subtilis and provides resistance against bacteriophage SPP1 infection. The high efficiency of the acquired immunity against phage could be useful in generation of biotechnologically important B. subtilis strains with engineered chromosomes. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 20% |
| Netherlands | 1 | 20% |
| France | 1 | 20% |
| Unknown | 2 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 60% |
| Scientists | 2 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 1% |
| China | 1 | 1% |
| Unknown | 79 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 21% |
| Student > Master | 15 | 19% |
| Student > Ph. D. Student | 13 | 16% |
| Student > Bachelor | 8 | 10% |
| Student > Doctoral Student | 2 | 2% |
| Other | 8 | 10% |
| Unknown | 18 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 26 | 32% |
| Agricultural and Biological Sciences | 21 | 26% |
| Immunology and Microbiology | 4 | 5% |
| Medicine and Dentistry | 3 | 4% |
| Engineering | 2 | 2% |
| Other | 3 | 4% |
| Unknown | 22 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 November 2017.
All research outputs
#16,048,318
of 25,374,917 outputs
Outputs from Journal of Industrial Microbiology & Biotechnology
#1,248
of 1,612 outputs
Outputs of similar age
#230,465
of 381,029 outputs
Outputs of similar age from Journal of Industrial Microbiology & Biotechnology
#14
of 26 outputs
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