| Title |
Nano electrospray combined with a quadrupole ion trap for the analysis of peptides and protein digests
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, February 1996
|
| DOI | 10.1016/1044-0305(95)00626-5 |
| Pubmed ID | |
| Authors |
Roman Körner, Matthias Wilm, Kenneth Morand, Michael Schubert, Matthias Mann |
| Abstract |
A nano electrospray (NanoES)/ion trap combination was developed to take advantage of the long spraying time of the NanoES source (Wilm, M. S., Mann, M. Int. J. Mass Spectrom. Ion Processes 1994, 136, 167-180) for extensive experiments on the ion trap. The low flow rate associated with the NanoES allows for optimization of experimental conditions and data acquisition for more than 30 min with 1 µL of solution. Thus, even time-consuming experiments, such as multiple fragmentation steps or the sequencing of many components in a mixture, can be performed with very small volumes of sample. Stored waveform inverse Fourier transformation (SWIFT) signals were used during the injection period to accumulate ions of low intensity and to improve the dynamic range of the quadrupole ion trap. To sequence peptides, a combination of nozzle-skimmer fragmentation, SWIFT accumulation of a single fragment to high intensity, and a second fragmentation step was employed to obtain complete sequence information. Unseparated peptide mixtures were infused and weak portions of the spectrum were enhanced by using the SWIFT method, followed by fragmentation of various accumulated peptide ions. |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 38 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 26% |
| Researcher | 7 | 18% |
| Student > Master | 5 | 13% |
| Student > Bachelor | 4 | 10% |
| Unspecified | 4 | 10% |
| Other | 8 | 21% |
| Unknown | 1 | 3% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 17 | 44% |
| Agricultural and Biological Sciences | 7 | 18% |
| Unspecified | 4 | 10% |
| Biochemistry, Genetics and Molecular Biology | 2 | 5% |
| Engineering | 2 | 5% |
| Other | 6 | 15% |
| Unknown | 1 | 3% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2022.
All research outputs
#5,446,629
of 25,373,627 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#555
of 3,833 outputs
Outputs of similar age
#7,025
of 81,538 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#2
of 13 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,833 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 81,538 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.