| Title |
A simple PCR condition for detection of a single cyst of Acanthamoeba species
|
|---|---|
| Published in |
Parasitology Research, October 2011
|
| DOI | 10.1007/s00436-011-2662-3 |
| Pubmed ID | |
| Authors |
Porntip Laummaunwai, Wipaporn Ruangjirachuporn, Thidarut Boonmars |
| Abstract |
Acanthamoeba is a free-living protozoan with a worldwide distribution in a variety of natural and artificial habitats. It has even been found in contact lens solution. Acanthamoeba spp. can cause infections such as granulomatous amoebic encephalitis and amoebic keratitis. Specific and sensitive diagnosis of Acanthamoeba infections can prevent clinical symptoms from worsening. Recently, PCR technique has been used for Acanthamoeba diagnosis. Unfortunately the dormant cyst of Acanthamoeba is resistant to chemical reagents; thus, most extraction of DNA uses a commercial DNA extraction kit for obtaining DNA for further use in polymerase chain reaction (PCR). Therefore in the present study, we improved the ability to diagnose Acanthamoeba using a simplified PCR technique. Interestingly, heating at 94°C for 10 min could release DNA which is amplified with specific primers designed from 16S rRNA. The PCR product is about 180 bp. This technique is a simple and efficient method for detection of Acanthamoeba-even a single cyst-and does not require high-cost reagents or complicated procedures to extract DNA. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 38% |
| Researcher | 4 | 15% |
| Professor > Associate Professor | 3 | 12% |
| Student > Ph. D. Student | 3 | 12% |
| Other | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 1 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 8 | 31% |
| Agricultural and Biological Sciences | 4 | 15% |
| Immunology and Microbiology | 4 | 15% |
| Biochemistry, Genetics and Molecular Biology | 3 | 12% |
| Environmental Science | 3 | 12% |
| Other | 1 | 4% |
| Unknown | 3 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 July 2012.
All research outputs
#20,161,674
of 22,671,366 outputs
Outputs from Parasitology Research
#2,864
of 3,768 outputs
Outputs of similar age
#125,030
of 135,981 outputs
Outputs of similar age from Parasitology Research
#23
of 25 outputs
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So far Altmetric has tracked 3,768 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.