| Title |
Nanoantibodies for detection and blocking of bioactivity of human vascular endothelial growth factor a165
|
|---|---|
| Published in |
Biochemistry, June 2012
|
| DOI | 10.1134/s0006297912060132 |
| Pubmed ID | |
| Authors |
S. V. Tillib, T. I. Ivanova, E. Yu. Lyssuk, S. S. Larin, A. V. Kibardin, E. V. Korobko, P. N. Vikhreva, N. V. Gnuchev, G. P. Georgiev, I. V. Korobko |
| Abstract |
Nanoantibodies (single-domain antibodies, nanobodies) derived from noncanonical single-chain immunoglobulins provide an attractive tool for in vitro and in vivo diagnostics as well as for development of targeted drugs for clinical use. Nanoantibodies against several clinically important targets have been developed and are actively investigated. However, no development of nanoantibodies against vascular endothelial growth factor VEGF-A(165) has been reported. We describe here the generation of nanoantibodies derived from single-chain Bactrian camel immunoglobulins directed against VEGF-A(165). We demonstrate that these nanoantibodies are suitable for enzyme-linked immunoassay to quantify human VEGF-A(165) as well as for blocking its activity. Our results provide a basis for diagnostic kit development for quantification of VEGF-A(165), which emerges as a biomarker useful in various pathological conditions. In addition, the nanoantibodies might be used for development of therapeutic molecules targeting VEGF-A(165)-dependent pathological neoangiogenesis. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 5% |
| Unknown | 18 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 32% |
| Professor | 5 | 26% |
| Student > Ph. D. Student | 2 | 11% |
| Student > Master | 2 | 11% |
| Unspecified | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 2 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 32% |
| Agricultural and Biological Sciences | 4 | 21% |
| Veterinary Science and Veterinary Medicine | 2 | 11% |
| Unspecified | 1 | 5% |
| Immunology and Microbiology | 1 | 5% |
| Other | 2 | 11% |
| Unknown | 3 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 July 2012.
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#22,759,452
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Outputs from Biochemistry
#21,449
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#164,286
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Outputs of similar age from Biochemistry
#75
of 84 outputs
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