Peptide and Protein Engineering

Chapter 1 SEA-Mediated Ligation Is Accelerated at Mildly Acidic pH: Application to the Formation of Difficult Peptide Junctions

Chapter 2 The Problem of Aspartimide Formation During Protein Chemical Synthesis Using SEA-Mediated Ligation

Chapter 3 Using the Interactive Tool of the Protein Chemical Synthesis Database

Chapter 4 Only a “Click” Away: Development of Arginine-Rich Peptide-Based Materials Using Click Chemistry

Chapter 5 Fluoroglycoproteins by Copper-Free Strain-Promoted Azide–Alkyne Cycloaddition

Chapter 6 Hybrid Silylated Peptides for the Design of Bio-functionalized Materials

Chapter 7 Synthesis of Peptide-Oligoethylene Glycol (OEG) Conjugates for Multivalent Modification of Nanomaterials

Chapter 8 Phage Display Methodologies

Chapter 9 Whole-Bacterium Ribosome Display Selection for Isolation of Antibacterial Affitins

Chapter 10 CIS Display: DNA-Based Technology as a Platform for Discovery of Therapeutic Biologics

Chapter 11 Building Scarless Gene Libraries in the Chromosome of Bacteria

Chapter 12 Streptavidin (Sav)-Based Artificial Metalloenzymes: Cofactor Design Considerations and Large-Scale Expression of Host Protein Variants

Chapter 13 Integrated Bacterial Production and Functional Screening of Expanded Cyclic Peptide Libraries for Identifying Chemical Rescuers of Pathogenic Protein Misfolding and Aggregation

Chapter 14 Site-Specific Incorporation of Non-canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli