| Title |
A novel method of selective removal of human DNA improves PCR sensitivity for detection of Salmonella Typhi in blood samples
|
|---|---|
| Published in |
BMC Infectious Diseases, July 2012
|
| DOI | 10.1186/1471-2334-12-164 |
| Pubmed ID | |
| Authors |
Liqing Zhou, Andrew J Pollard |
| Abstract |
Enteric fever is a major public health problem, causing an estimated 21million new cases and 216,000 or more deaths every year. Current diagnosis of the disease is inadequate. Blood culture only identifies 45 to 70% of the cases and is time-consuming. Serological tests have very low sensitivity and specificity. Clinical samples obtained for diagnosis of enteric fever in the field generally have <1 organism/ml of blood, so that even PCR-based methods, widely used for detection of other infectious diseases, are not a straightforward option in typhoid diagnosis. We developed a novel method to enrich target bacterial DNA by selective removal of human DNA from blood samples, enhancing the sensitivity of PCR tests. This method offers the possibility of improving PCR assays directly using clinical specimens for diagnosis of this globally important infectious disease. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | <1% |
| Germany | 1 | <1% |
| Luxembourg | 1 | <1% |
| Unknown | 99 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 27 | 26% |
| Researcher | 26 | 25% |
| Student > Postgraduate | 9 | 9% |
| Student > Master | 9 | 9% |
| Student > Bachelor | 7 | 7% |
| Other | 15 | 15% |
| Unknown | 9 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 43 | 42% |
| Medicine and Dentistry | 19 | 19% |
| Biochemistry, Genetics and Molecular Biology | 16 | 16% |
| Immunology and Microbiology | 12 | 12% |
| Engineering | 2 | 2% |
| Other | 1 | <1% |
| Unknown | 9 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2019.
All research outputs
#4,571,727
of 22,671,366 outputs
Outputs from BMC Infectious Diseases
#1,480
of 7,641 outputs
Outputs of similar age
#32,106
of 164,673 outputs
Outputs of similar age from BMC Infectious Diseases
#13
of 76 outputs
Altmetric has tracked 22,671,366 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 7,641 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.6. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 164,673 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.