Ribozymes

Chapter 1 Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing In Vivo

Chapter 2 Co-transcriptional Analysis of Self-Cleaving Ribozymes and Their Ligand Dependence

Chapter 3 Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediates

Chapter 4 Demonstration of a Ribozyme in Epsilon Domain of Hepatitis B Virus RNA

Chapter 5 In Vitro Selection of Varkud Satellite Ribozyme Variants that Cleave a Modified Stem-Loop Substrate

Chapter 6 Characterization and Optimization of a Deoxyribozyme with a Short Left Binding Arm

Chapter 7 Computer-Aided Design of Active Pseudoknotted Hammerhead Ribozymes

Chapter 8 Inverse RNA Folding Workflow to Design and Test Ribozymes that Include Pseudoknots

Chapter 9 Using an L7Ae-Tethered, Hydroxyl Radical-Mediated Footprinting Strategy to Identify and Validate Kink-Turns in RNAs

Chapter 10 SHAPE Profiling to Probe Group II Intron Conformational Dynamics During Splicing

Chapter 11 Dynamics-Function Analysis in Catalytic RNA Using NMR Spin Relaxation and Conformationally Restricted Nucleotides

Chapter 12 Design and Evaluation of Guide RNA Transcripts with a 3′-Terminal HDV Ribozyme to Enhance CRISPR-Based Gene Inactivation

Chapter 13 Design and Evaluation of AgoshRNAs with 3′-Terminal HDV Ribozymes to Enhance the Silencing Activity

Chapter 14 Cloning and Detection of Aptamer-Ribozyme Conjugations

Chapter 15 Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo

Chapter 16 Long Non-coding RNA Depletion Using Self-Cleaving Ribozymes