| Title |
Ten commercial antibodies for alpha-1-adrenergic receptor subtypes are nonspecific
|
|---|---|
| Published in |
Naunyn-Schmiedeberg's Archives of Pharmacology, November 2008
|
| DOI | 10.1007/s00210-008-0368-6 |
| Pubmed ID | |
| Authors |
Brian C. Jensen, Philip M. Swigart, Paul C. Simpson |
| Abstract |
Commercial antibodies are used widely to quantify and localize the alpha1-adrenergic receptor (AR) subtypes, alpha1A, alpha1B, and alpha1D. We tested ten antibodies, from abcam and Santa Cruz, using western blot with heart and brain tissue from wild-type (WT) mice and mice with systemic knockout (KO) of one or all three subtypes. We found that none of the antibodies detected a band in WT that was absent in the appropriate KO or in the KO that was null for all alpha1-ARs (ABDKO). We conclude that the antibodies we tested are not specific for alpha1-ARs. These results raise caution with prior studies using these reagents. For now, competition radioligand binding is the only reliable approach to quantify the alpha1-AR subtype proteins. Receptor protein localization remains a challenge. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 3% |
| Japan | 1 | 1% |
| Denmark | 1 | 1% |
| Unknown | 71 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 23% |
| Researcher | 15 | 20% |
| Student > Master | 7 | 9% |
| Student > Doctoral Student | 6 | 8% |
| Professor > Associate Professor | 5 | 7% |
| Other | 12 | 16% |
| Unknown | 13 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 32% |
| Biochemistry, Genetics and Molecular Biology | 11 | 15% |
| Medicine and Dentistry | 9 | 12% |
| Neuroscience | 8 | 11% |
| Chemistry | 2 | 3% |
| Other | 7 | 9% |
| Unknown | 14 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 April 2018.
All research outputs
#13,365,440
of 22,673,450 outputs
Outputs from Naunyn-Schmiedeberg's Archives of Pharmacology
#1,276
of 1,719 outputs
Outputs of similar age
#75,644
of 91,952 outputs
Outputs of similar age from Naunyn-Schmiedeberg's Archives of Pharmacology
#5
of 6 outputs
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