| Title |
In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus
|
|---|---|
| Published in |
Parasitology Research, June 2016
|
| DOI | 10.1007/s00436-016-5166-3 |
| Pubmed ID | |
| Authors |
Fen Wang, Bin Ye |
| Abstract |
Cystic echinococcosis is a worldwide zoonosis caused by Echinococcus granulosus. Because the methods of diagnosis and treatment for cystic echinococcosis were limited, it is still necessary to screen target proteins for the development of new anti-hydatidosis vaccine. In this study, the triosephosphate isomerase gene of E. granulosus was in silico cloned. The B cell and T cell epitopes were predicted by bioinformatics methods. The cDNA sequence of EgTIM was composition of 1094 base pairs, with an open reading frame of 753 base pairs. The deduced amino acid sequences were composed of 250 amino acids. Five cross-reactive epitopes, locating on 21aa-35aa, 43aa-57aa, 94aa-107aa, 115-129aa, and 164aa-183aa, could be expected to serve as candidate epitopes in the development of vaccine against E. granulosus. These results could provide bases for gene cloning, recombinant expression, and the designation of anti-hydatidosis vaccine. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 19% |
| Researcher | 3 | 14% |
| Student > Bachelor | 2 | 10% |
| Student > Postgraduate | 2 | 10% |
| Student > Master | 2 | 10% |
| Other | 1 | 5% |
| Unknown | 7 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 14% |
| Biochemistry, Genetics and Molecular Biology | 3 | 14% |
| Immunology and Microbiology | 2 | 10% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Chemistry | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 11 | 52% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 May 2017.
All research outputs
#19,015,492
of 23,577,654 outputs
Outputs from Parasitology Research
#2,421
of 3,843 outputs
Outputs of similar age
#260,737
of 344,946 outputs
Outputs of similar age from Parasitology Research
#58
of 102 outputs
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