| Title |
TM7SF1 (GPR137B): a novel lysosome integral membrane protein
|
|---|---|
| Published in |
Molecular Biology Reports, June 2012
|
| DOI | 10.1007/s11033-012-1755-0 |
| Pubmed ID | |
| Authors |
Jialin Gao, Libin Xia, Meiqing Lu, Binhua Zhang, Yueping Chen, Rang Xu, Lizhuo Wang |
| Abstract |
In the previous proteomic study of human placenta, transmembrane 7 superfamily member 1 (TM7SF1) was found enriched in lysosome compartments. TM7SF1 encodes a 399-amino acid protein with a calculated molecular mass of 45 kDa. Bioinformatic analysis of its amino acid sequence showed that it is a multipass transmembrane protein containing a potential dileucine-based lysosomal targeting signal and four putative N-glycosylation sites. By percoll-gradient centrifugation and further subfraction ways, the lysosomal solute and membrane compartments were isolated respectively. Immunoblotting analysis indicated that TM7SF1 was co-fractioned with lysosome associated membrane protein 2 (LAMP2), which was only detected in lysosomal membrane compartments whereas not detected in the solute compartments. Using specific anti-TM7SF1 antibody and double-immunofluorescence with lysosome membrane protein LAMP1 and Lyso-Tracker Red, the colocalisations of endogenous TM7SF1 with lysosome and late endosome markers were demonstrated. All of this indicated that TM7SF1 is an integral lysosome membrane protein. Rat ortholog of TM7SF1 was found to be strongly expressed in heart, liver, kidney and brain while not or low detected in other tissues. In summary, TM7SF1 was a lysosomal integral membrane protein that shows tissue-specific expression. As a G-protein-coupled receptor in lysosome membrane, TM7SF1 was predicted function as signal transduction across lysosome membrane. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 50% |
| United States | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 6% |
| Unknown | 16 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 18% |
| Student > Ph. D. Student | 2 | 12% |
| Student > Master | 2 | 12% |
| Other | 1 | 6% |
| Professor | 1 | 6% |
| Other | 2 | 12% |
| Unknown | 6 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 18% |
| Agricultural and Biological Sciences | 3 | 18% |
| Medicine and Dentistry | 3 | 18% |
| Immunology and Microbiology | 1 | 6% |
| Neuroscience | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 6 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2012.
All research outputs
#15,248,503
of 22,673,450 outputs
Outputs from Molecular Biology Reports
#1,112
of 2,873 outputs
Outputs of similar age
#104,813
of 164,533 outputs
Outputs of similar age from Molecular Biology Reports
#23
of 48 outputs
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