| Title |
Co-lyophilized Aspirin with Trehalose Causes Less Injury to Human Gastric Cells and Gastric Mucosa of Rats
|
|---|---|
| Published in |
Digestive Diseases and Sciences, May 2016
|
| DOI | 10.1007/s10620-016-4209-z |
| Pubmed ID | |
| Authors |
Lee-Shuan Lin, Yuko Kayasuga-Kariya, Shugo Nakamura, Nobuyuki Shimohata, Takamasa Sakai, Ayano Fujisawa, Yuki Akagi, Shigeki Suzuki, Ung-il Chung, Nobuo Sasaki, Manabu Mochizuki |
| Abstract |
Aspirin is one of the most popular NSAIDs worldwide because of its anti-inflammatory and anticoagulant effects, and however, gastrointestinal injury remains a major complication. We previously reported co-lyophilized aspirin/trehalose (Lyo A/T) decreased the aspirin-induced gastric lesions in dogs. This study investigated the mechanism of gastroprotective effects of trehalose in vitro and in vivo. The apoptotic assays were performed in a human gastric carcinoma cell line, which was treated with aspirin, mixed aspirin/trehalose (Mix A/T) or Lyo A/T. Gastric ulcer severity was examined after oral administration of drugs in rats. In addition, the mucosal tissue apoptotic status in drug-treated rats was evaluated. Molecular dynamics simulations and laser Raman spectroscopy were performed in order to examine the molecular properties of Lyo A/T. DNA fragmentation was detected in AGS cells that were treated with aspirin and Mix A/T, but not in the Lyo A/T-treated cells. There were fewer apoptotic cells in the Lyo A/T-treated cells than in the other cells. Gastric injury was reduced in rats that received oral Lyo A/T compared with the others, while PGE2 synthesis was equally decreased in all groups. TUNEL assay and immunohistochemistry of cleaved caspase-3 in the mucosal tissues also revealed that Lyo A/T treatment induced less apoptosis than the others. The Lyo A/T spectrum showed clear differences in several Raman bands compared with that of Mix A/T. Our data showed that co-lyophilization of aspirin with trehalose reduced gastric injury, potentially through suppression of aspirin-induced mucosal cell apoptosis while retaining its anti-inflammatory effects. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 31% |
| Professor > Associate Professor | 4 | 25% |
| Student > Bachelor | 2 | 13% |
| Other | 1 | 6% |
| Researcher | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 4 | 25% |
| Medicine and Dentistry | 4 | 25% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Biochemistry, Genetics and Molecular Biology | 1 | 6% |
| Agricultural and Biological Sciences | 1 | 6% |
| Other | 3 | 19% |
| Unknown | 2 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 June 2016.
All research outputs
#21,358,731
of 23,854,458 outputs
Outputs from Digestive Diseases and Sciences
#3,790
of 4,304 outputs
Outputs of similar age
#299,173
of 343,228 outputs
Outputs of similar age from Digestive Diseases and Sciences
#34
of 38 outputs
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