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Leucocytes

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Cover of 'Leucocytes'

Table of Contents

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    Book Overview
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    Chapter 1 ENU-Based Phenotype-Driven Screening
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    Chapter 2 Detection and quantification of cytokines and other biomarkers.
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    Chapter 3 Flow cytometry analysis of cell cycling and proliferation in mouse hematopoietic stem and progenitor cells.
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    Chapter 4 Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow In Vivo
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    Chapter 5 Analyzing Cell Death Events in Cultured Leukocytes
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    Chapter 6 Leucocytes
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    Chapter 7 Isolation of Human and Mouse Neutrophils Ex Vivo and In Vitro
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    Chapter 8 Measurement of Oxidative Burst in Neutrophils
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    Chapter 9 Measurement of Neutrophil Elastase, Proteinase 3, and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic Substrates
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    Chapter 10 The Macrophage
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    Chapter 11 Generation and Characterization of MacGreen Mice, the Cfs1r -EGFP Transgenic Mice
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    Chapter 12 Generation of Mouse Bone Marrow-Derived Macrophages
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    Chapter 13 Leucocytes
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    Chapter 14 In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages
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    Chapter 15 Measuring the Inflammasome
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    Chapter 16 Arginine and Macrophage Activation
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    Chapter 17 Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation
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    Chapter 18 Detection of Human and Mouse Granzyme B Activity in Cell Extracts
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    Chapter 19 Leucocytes
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    Chapter 20 Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry
Attention for Chapter 3: Flow cytometry analysis of cell cycling and proliferation in mouse hematopoietic stem and progenitor cells.
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Chapter title
Flow cytometry analysis of cell cycling and proliferation in mouse hematopoietic stem and progenitor cells.
Chapter number 3
Book title
Leucocytes
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-527-5_3
Pubmed ID
Book ISBNs
978-1-61779-526-8, 978-1-61779-527-5
Authors

Valérie Barbier, Bianca Nowlan, Jean-Pierre Lévesque, Ingrid G. Winkler, Barbier, Valérie, Nowlan, Bianca, Lévesque, Jean-Pierre, Winkler, Ingrid G.

Abstract

The hematopoietic system is highly proliferative in the bone marrow (BM) due to the short half-life of granulocytes and platelets in the blood. Analysis of cell cycling and cell proliferation in vivo in specific populations of the mouse BM has highlighted some key properties of adult hematopoietic stem cells (HSCs). For instance, despite their enormous proliferation and repopulation potential, most true HSC are deeply quiescent in G(0) phase of the cell cycle and divide very infrequently, while less potent lineage-restricted progenitors divide rapidly to replace the daily consumption of blood leukocytes, erythrocytes, and platelets. In response to stress, e.g., following ablative chemotherapy or irradiation, HSC must enter the cell cycle to rapidly repopulate the BM with progenitors. Due to their extreme rarity in the BM, at least five color flow cytometry for cell surface antigens has to be combined with staining for DNA content and nuclear markers of proliferation to analyze cell cycle and proliferation of HSC in vivo. In this chapter, we describe two methods to stain mouse HSC to (1) distinguish all phases of the cell cycle (G(0), G(1), S, and G(2)/M) and (2) analyze the divisional history of HSC in vivo by incorporation of the thymidine analog 5-bromo-2-deoxyuridine.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 4%
Unknown 27 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 25%
Student > Master 6 21%
Student > Ph. D. Student 6 21%
Student > Bachelor 4 14%
Student > Doctoral Student 2 7%
Other 1 4%
Unknown 2 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 64%
Biochemistry, Genetics and Molecular Biology 2 7%
Medicine and Dentistry 2 7%
Immunology and Microbiology 1 4%
Nursing and Health Professions 1 4%
Other 2 7%
Unknown 2 7%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 March 2012.
All research outputs
#20,163,398
of 22,673,450 outputs
Outputs from Methods in molecular biology
#9,820
of 13,037 outputs
Outputs of similar age
#222,712
of 246,111 outputs
Outputs of similar age from Methods in molecular biology
#418
of 471 outputs
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So far Altmetric has tracked 13,037 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 471 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.