| Title |
Isothiocyanates induce oxidative stress and suppress the metastasis potential of human non-small cell lung cancer cells
|
|---|---|
| Published in |
BMC Cancer, June 2010
|
| DOI | 10.1186/1471-2407-10-269 |
| Pubmed ID | |
| Authors |
Xiang Wu, Yu Zhu, Huiqin Yan, Boning Liu, Ying Li, Qinghua Zhou, Ke Xu |
| Abstract |
Isothiocyanates are natural compounds found in consumable cruciferous vegetables. They have been shown to inhibit chemical carcinogenesis by a wide variety of chemical carcinogens in animal models. Recent studies have also shown that isothiocyanates have antitumor activity, inhibiting the growth of several types of cultured human cancer cells. Our previous study showed that PEITC inhibited human leukemia cells growth by inducing apoptosis. However, the effect of isothiocyanates on lung cancer cell metastasis has not been studied. In the present study, we investigated the inhibitory effects of BITC and PEITC on metastatic potential of highly metastatic human lung cancer L9981 cells. Cell migration and invasion were measured by wound healing assay and transwell chemotaxis assay. Expression of metastasis-related genes was assessed by quantitative RT-PCR and Western blotting. The mechanisms of action were evaluated by flow cytometry, reporter assay and Western blotting. Our data showed that both BITC and PEITC inhibited L9981 cell growth in a dose-dependent manner, the IC50 values were 5.0 and 9.7 microM, respectively. Cell migrations were reduced to 8.1% and 16.5% of control, respectively; and cell invasions were reduced to 2.7% and 7.3% of control, respectively. Metastasis-related genes MMP-2, Twist and beta-catenin were also modulated. BITC and PEITC inhibited cell survival signaling molecules Akt and NFkappaB activation. Moreover, BITC and PEITC increased ROS generation and caused GSH depletion. Pretreatment with NAC blocked BITC and PEITC induced ROS elevation and NFkappaB inhibition. Our results indicated that BITC and PEITC suppress lung cancer cell metastasis potential by modulation of metastasis-related gene expression, inhibition of Akt/NFkappaB pathway. Induction of oxidative stress may play an important role. |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Portugal | 1 | 2% |
| China | 1 | 2% |
| Unknown | 62 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 13 | 20% |
| Student > Bachelor | 10 | 15% |
| Researcher | 8 | 12% |
| Student > Ph. D. Student | 8 | 12% |
| Student > Doctoral Student | 4 | 6% |
| Other | 7 | 11% |
| Unknown | 15 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 22% |
| Biochemistry, Genetics and Molecular Biology | 8 | 12% |
| Chemistry | 8 | 12% |
| Medicine and Dentistry | 5 | 8% |
| Psychology | 3 | 5% |
| Other | 5 | 8% |
| Unknown | 22 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 June 2016.
All research outputs
#7,239,860
of 22,877,793 outputs
Outputs from BMC Cancer
#1,948
of 8,325 outputs
Outputs of similar age
#33,335
of 96,300 outputs
Outputs of similar age from BMC Cancer
#22
of 68 outputs
Altmetric has tracked 22,877,793 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 8,325 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 96,300 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 68 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.