Title |
Development of internally controlled duplex real-time NASBA diagnostics assays for the detection of microorganisms associated with bacterial meningitis
|
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Published in |
Journal of Microbiological Methods, June 2016
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DOI | 10.1016/j.mimet.2016.06.017 |
Pubmed ID | |
Authors |
Eoin Clancy, Helena Coughlan, Owen Higgins, Teck Wee Boo, Martin Cormican, Louise Barrett, Terry J Smith, Kate Reddington, Thomas Barry |
Abstract |
Three duplex molecular beacon based real-time Nucleic Acid Sequence Based Amplification (NASBA) assays have been designed and experimentally validated targeting RNA transcripts for the detection and identification of Haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae respectively. Each real-time NASBA diagnostics assay includes an endogenous non-competitive Internal Amplification Control (IAC) to amplify the splice variant 1 mRNA of the Homo sapiens TBP gene from human total RNA. All three duplex real-time NASBA diagnostics assays were determined to be 100% specific for the target species tested for. Also the Limits of Detection (LOD) for the H. influenzae, N. meningitidis and S. pneumoniae duplex real-time NASBA assays were 55.36, 0.99, and 57.24 Cell Equivalents (CE) respectively. These robust duplex real-time NASBA diagnostics assays have the potential to be used in a clinical setting for the rapid (<60 min) specific detection and identification of the most prominent microorganisms associated with bacterial meningitis in humans. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Ireland | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 15% |
Student > Master | 4 | 15% |
Researcher | 4 | 15% |
Student > Ph. D. Student | 4 | 15% |
Other | 1 | 4% |
Other | 2 | 8% |
Unknown | 7 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 23% |
Medicine and Dentistry | 5 | 19% |
Agricultural and Biological Sciences | 3 | 12% |
Immunology and Microbiology | 3 | 12% |
Chemistry | 1 | 4% |
Other | 0 | 0% |
Unknown | 8 | 31% |