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Development of internally controlled duplex real-time NASBA diagnostics assays for the detection of microorganisms associated with bacterial meningitis

Overview of attention for article published in Journal of Microbiological Methods, June 2016
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Title
Development of internally controlled duplex real-time NASBA diagnostics assays for the detection of microorganisms associated with bacterial meningitis
Published in
Journal of Microbiological Methods, June 2016
DOI 10.1016/j.mimet.2016.06.017
Pubmed ID
Authors

Eoin Clancy, Helena Coughlan, Owen Higgins, Teck Wee Boo, Martin Cormican, Louise Barrett, Terry J Smith, Kate Reddington, Thomas Barry

Abstract

Three duplex molecular beacon based real-time Nucleic Acid Sequence Based Amplification (NASBA) assays have been designed and experimentally validated targeting RNA transcripts for the detection and identification of Haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae respectively. Each real-time NASBA diagnostics assay includes an endogenous non-competitive Internal Amplification Control (IAC) to amplify the splice variant 1 mRNA of the Homo sapiens TBP gene from human total RNA. All three duplex real-time NASBA diagnostics assays were determined to be 100% specific for the target species tested for. Also the Limits of Detection (LOD) for the H. influenzae, N. meningitidis and S. pneumoniae duplex real-time NASBA assays were 55.36, 0.99, and 57.24 Cell Equivalents (CE) respectively. These robust duplex real-time NASBA diagnostics assays have the potential to be used in a clinical setting for the rapid (<60 min) specific detection and identification of the most prominent microorganisms associated with bacterial meningitis in humans.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 15%
Student > Master 4 15%
Researcher 4 15%
Student > Ph. D. Student 4 15%
Other 1 4%
Other 2 8%
Unknown 7 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 23%
Medicine and Dentistry 5 19%
Agricultural and Biological Sciences 3 12%
Immunology and Microbiology 3 12%
Chemistry 1 4%
Other 0 0%
Unknown 8 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2016.
All research outputs
#17,285,668
of 25,374,647 outputs
Outputs from Journal of Microbiological Methods
#1,683
of 2,350 outputs
Outputs of similar age
#228,351
of 353,557 outputs
Outputs of similar age from Journal of Microbiological Methods
#32
of 58 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,350 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,557 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.