| Title |
High-Frequency Electron Paramagnetic Resonance Spectroscopy of Nitroxide-Functionalized Nanodiamonds in Aqueous Solution
|
|---|---|
| Published in |
Cell Biochemistry and Biophysics, June 2016
|
| DOI | 10.1007/s12013-016-0739-4 |
| Pubmed ID | |
| Authors |
R. D. Akiel, V. Stepanov, S. Takahashi |
| Abstract |
Nanodiamond (ND) is an attractive class of nanomaterial for fluorescent labeling, magnetic sensing of biological molecules, and targeted drug delivery. Many of those applications require tethering of target biological molecules on the ND surface. Even though many approaches have been developed to attach macromolecules to the ND surface, it remains challenging to characterize dynamics of tethered molecule. Here, we show high-frequency electron paramagnetic resonance (HF EPR) spectroscopy of nitroxide-functionalized NDs. Nitroxide radical is a commonly used spin label to investigate dynamics of biological molecules. In the investigation, we developed a sample holder to overcome water absorption of HF microwave. Then, we demonstrated HF EPR spectroscopy of nitroxide-functionalized NDs in aqueous solution and showed clear spectral distinction of ND and nitroxide EPR signals. Moreover, through EPR spectral analysis, we investigate dynamics of nitroxide radicals on the ND surface. The demonstration sheds light on the use of HF EPR spectroscopy to investigate biological molecule-functionalized nanoparticles. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Australia | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 29% |
| Student > Doctoral Student | 2 | 14% |
| Professor | 2 | 14% |
| Student > Bachelor | 2 | 14% |
| Researcher | 2 | 14% |
| Other | 1 | 7% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 5 | 36% |
| Chemistry | 4 | 29% |
| Immunology and Microbiology | 1 | 7% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Materials Science | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 1 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 May 2017.
All research outputs
#14,204,359
of 22,879,161 outputs
Outputs from Cell Biochemistry and Biophysics
#311
of 911 outputs
Outputs of similar age
#200,570
of 353,105 outputs
Outputs of similar age from Cell Biochemistry and Biophysics
#5
of 25 outputs
Altmetric has tracked 22,879,161 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 911 research outputs from this source. They receive a mean Attention Score of 2.0. This one has gotten more attention than average, scoring higher than 65% of its peers.
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We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.