Title |
A replication-time-controlling sequence element in Schizosaccharomyces pombe
|
---|---|
Published in |
Chromosoma, June 2016
|
DOI | 10.1007/s00412-016-0606-5 |
Pubmed ID | |
Authors |
Vishnu P. Tripathi, Dharani D. Dubey |
Abstract |
Eukaryotic replication origins are highly variable in their activity and replication timing. The nature and role of cis-acting regulatory sequences that control chromosomal replication timing is not well defined. In the fission yeast, Schizosaccharomyces pombe, a 200-bp late-replication-enforcing element (LRE), has been shown to enforce late replication of ARS elements in plasmids. Here, we show that a short (133-bp) fragment of the LRE (shLRE) is required for causing late replication of adjoining origins in its native as well as in an ectopic early-replicating chromosomal location. Active from both sides of an early-replicating origin, the shLRE is a bona fide cis-acting regulatory element that imposes late replication timing in the chromosome. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
France | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 27% |
Student > Ph. D. Student | 2 | 18% |
Student > Bachelor | 1 | 9% |
Professor | 1 | 9% |
Student > Master | 1 | 9% |
Other | 1 | 9% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 45% |
Agricultural and Biological Sciences | 3 | 27% |
Immunology and Microbiology | 1 | 9% |
Unknown | 2 | 18% |