| Title |
Epigenetic silencing of TPM2 contributes to colorectal cancer progression upon RhoA activation
|
|---|---|
| Published in |
Tumor Biology, June 2016
|
| DOI | 10.1007/s13277-016-5103-1 |
| Pubmed ID | |
| Authors |
Ji Cui, Yonghua Cai, Ying Hu, Zenghong Huang, Yanxin Luo, Andrew M. Kaz, Zihuan Yang, Dianke Chen, Xinjuan Fan, William M. Grady, Jianping Wang |
| Abstract |
Beta-tropomyosin (β-tropomyosin, TPM2) has been found to be downregulated in colorectal cancer (CRC) in previous studies. In this study, we aimed to investigate the mechanisms and potential biological consequences of the downregulation of TPM2 in colorectal cancer. TPM2 expression in colorectal cancer was assessed by qRT-PCR and immunostaining. The biological functions of TPM2 were assessed in cell lines either overexpressing or underexpressingTPM2. Aberrant DNA methylation in the promoter region is associated with suppression of TPM2 expression in primary colorectal cancer tissue samples. Treatment with the demethylation agent 5-AZA can induceTPM2 expression in colorectal cancer cell lines. Reconstitution of TPM2 suppresses cell proliferation and migration in colorectal cancer cell lines, whereas the loss of TPM2 expression is associated with increased tumor proliferation and migration in vitro, which was accompanied by RhoA activation. In summary, our findings indicate that TPM2 appears to be commonly silenced by aberrant DNA methylation in colon cancer. TPM2 loss is associated with RhoA activation and tumor proliferation. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 26% |
| Student > Ph. D. Student | 3 | 16% |
| Student > Bachelor | 2 | 11% |
| Student > Doctoral Student | 1 | 5% |
| Professor | 1 | 5% |
| Other | 2 | 11% |
| Unknown | 5 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 26% |
| Agricultural and Biological Sciences | 2 | 11% |
| Engineering | 2 | 11% |
| Medicine and Dentistry | 2 | 11% |
| Nursing and Health Professions | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 June 2016.
All research outputs
#20,334,427
of 22,879,161 outputs
Outputs from Tumor Biology
#1,835
of 2,623 outputs
Outputs of similar age
#305,332
of 352,801 outputs
Outputs of similar age from Tumor Biology
#60
of 92 outputs
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