| Title |
FPOP-LC-MS/MS Suggests Differences in Interaction Sites of Amphipols and Detergents with Outer Membrane Proteins
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, June 2016
|
| DOI | 10.1007/s13361-016-1421-1 |
| Pubmed ID | |
| Authors |
Thomas G. Watkinson, Antonio N. Calabrese, James R. Ault, Sheena E. Radford, Alison E. Ashcroft |
| Abstract |
Amphipols are a class of novel surfactants that are capable of stabilizing the native state of membrane proteins. They have been shown to be highly effective, in some cases more so than detergent micelles, at maintaining the structural integrity of membrane proteins in solution, and have shown promise as vehicles for delivering native membrane proteins into the gas phase for structural interrogation. Here, we use fast photochemical oxidation of proteins (FPOP), which irreversibly labels the side chains of solvent-accessible residues with hydroxyl radicals generated by laser photolysis of hydrogen peroxide, to compare the solvent accessibility of the outer membrane protein OmpT when solubilized with the amphipol A8-35 or with n-dodecyl-β-maltoside (DDM) detergent micelles. Using quantitative mass spectrometry analyses, we show that fast photochemical oxidation reveals differences in the extent of solvent accessibility of residues between the A8-35 and DDM solubilized states, providing a rationale for the increased stability of membrane proteins solubilized with amphipol compared with detergent micelles, as a result of additional intermolecular contacts. Graphical Abstract ᅟ. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 32 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 21% |
| Student > Ph. D. Student | 6 | 18% |
| Student > Master | 6 | 18% |
| Student > Bachelor | 3 | 9% |
| Other | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 8 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 13 | 39% |
| Biochemistry, Genetics and Molecular Biology | 6 | 18% |
| Agricultural and Biological Sciences | 4 | 12% |
| Unspecified | 1 | 3% |
| Chemical Engineering | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 8 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 June 2016.
All research outputs
#20,657,128
of 25,374,917 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#3,085
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Outputs of similar age
#284,211
of 368,661 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#14
of 26 outputs
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