Title |
A molecular biology and phase II study of imetelstat (GRN163L) in children with recurrent or refractory central nervous system malignancies: a pediatric brain tumor consortium study
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Published in |
Journal of Neuro-Oncology, June 2016
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DOI | 10.1007/s11060-016-2189-7 |
Pubmed ID | |
Authors |
Ralph Salloum, Trent R. Hummel, Shiva Senthil Kumar, Kathleen Dorris, Shaoyu Li, Tong Lin, Vinay M. Daryani, Clinton F. Stewart, Lili Miles, Tina Young Poussaint, Charles Stevenson, Stewart Goldman, Girish Dhall, Roger Packer, Paul Fisher, Ian F. Pollack, Maryam Fouladi, James Boyett, Rachid Drissi |
Abstract |
Telomerase activation is critical in many cancers including central nervous system (CNS) tumors. Imetelstat is an oligonucleotide that binds to the template region of the RNA component of telomerase, inhibiting its enzymatic activity. We conducted an investigator-sponsored molecular biology (MB) and phase II study to estimate inhibition of tumor telomerase activity and sustained responses by imetelstat in children with recurrent CNS malignancies. In the MB study, patients with recurrent medulloblastoma, high-grade glioma (HGG) or ependymoma undergoing resection received one dose of imetelstat as a 2-h intravenous infusion at 285 mg/m(2), 12-24 h before surgery. Telomerase activity was evaluated in fresh tumor from surgery. Post-surgery and in the phase II study, patients received imetelstat IV (days 1 and 8 q21-days) at 285 mg/m(2). Imetelstat pharmacokinetic and pharmacodynamic studies were performed. Of two evaluable patients on the MB trial, intratumoral telomerase activity was inhibited by 95 % compared to baseline archival tissue in one patient and was inevaluable in one patient. Forty-two patients (40 evaluable for toxicity) were enrolled: 9 medulloblastomas, 18 HGG, 4 ependymomas, 9 diffuse intrinsic pontine gliomas. Most common grade 3/4 toxicities included thrombocytopenia (32.5 %), lymphopenia (17.5 %), neutropenia (12.5 %), ALT (7.5 %) and AST (5 %) elevation. Two patients died of intratumoral hemorrhage secondary to thrombocytopenia leading to premature study closure. No objective responses were observed. Telomerase inhibition was observed in peripheral blood mononuclear cells (PBMCs) for at least 8 days. Imetelstat demonstrated intratumoral and PBMC target inhibition; the regimen proved too toxic in children with recurrent CNS tumors. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Portugal | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 1% |
France | 1 | 1% |
Unknown | 87 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 16 | 18% |
Researcher | 12 | 13% |
Student > Ph. D. Student | 10 | 11% |
Student > Master | 8 | 9% |
Student > Postgraduate | 5 | 6% |
Other | 12 | 13% |
Unknown | 26 | 29% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 24 | 27% |
Biochemistry, Genetics and Molecular Biology | 17 | 19% |
Agricultural and Biological Sciences | 5 | 6% |
Chemistry | 3 | 3% |
Immunology and Microbiology | 2 | 2% |
Other | 12 | 13% |
Unknown | 26 | 29% |