| Title |
Heavy Metal Resistances and Chromium Removal of a Novel Cr(VI)-Reducing Pseudomonad Strain Isolated from Circulating Cooling Water of Iron and Steel Plant
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, June 2016
|
| DOI | 10.1007/s12010-016-2170-0 |
| Pubmed ID | |
| Authors |
Jian-Kun Zhang, Zhen-Hua Wang, Yun Ye |
| Abstract |
Three bacterial isolates, GT2, GT3, and GT7, were isolated from the sludge and water of a circulating cooling system of iron and steel plant by screening on Cr(VI)-containing plates. Three isolates were characterized as the members of the genus Pseudomonas on the basis of phenotypic characteristics and 16S rRNA sequence analysis. All isolates were capable of resisting multiple antibiotics and heavy metals. GT7 was most resistant to Cr(VI), with a minimum inhibitory concentration (MIC) of 6.5 mmol L(-1). GT7 displayed varied rates of Cr(VI) reduction in M2 broth, which was dependent on pH, initial Cr(VI) concentration, and inoculating dose. Total chromium analysis revealed that GT7 could remove a part of chromium from the media, and the maximum rate of chromium removal was up to 40.8 %. The Cr(VI) reductase activity of GT7 was mainly associated with the soluble fraction of cell-free extracts and reached optimum at pH 6.0∼8.0. The reductase activity was apparently enhanced by external electron donors and Cu(II), whereas it was seriously inhibited by Hg(II), Cd(II), and Zn(II). The reductase showed a K m of 74 μmol L(-1) of Cr(VI) and a V max of 0.86 μmol of Cr(VI) min(-1) mg(-1) of protein. The results suggested that GT7 could be a promising candidate for in situ bioremediation of Cr(VI). |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 8 | 20% |
| Student > Ph. D. Student | 7 | 18% |
| Student > Master | 6 | 15% |
| Researcher | 2 | 5% |
| Lecturer > Senior Lecturer | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 13 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 20% |
| Agricultural and Biological Sciences | 4 | 10% |
| Chemistry | 3 | 8% |
| Environmental Science | 2 | 5% |
| Engineering | 2 | 5% |
| Other | 5 | 13% |
| Unknown | 16 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 June 2016.
All research outputs
#15,379,760
of 22,880,230 outputs
Outputs from Applied Biochemistry and Biotechnology
#1,557
of 2,509 outputs
Outputs of similar age
#223,009
of 352,119 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#15
of 37 outputs
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