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X Demographics
Mendeley readers
Attention Score in Context
| Title |
AAV-Mediated CRISPR/Cas Gene Editing of Retinal Cells In VivoAAV-Mediated CRISPR/Cas Gene Editing of Retinal Cells
|
|---|---|
| Published in |
Investigative Ophthalmology & Visual Science, June 2016
|
| DOI | 10.1167/iovs.16-19316 |
| Pubmed ID | |
| Authors |
Sandy S. C. Hung, Vicki Chrysostomou, Fan Li, Jeremiah K. H. Lim, Jiang-Hui Wang, Joseph E. Powell, Leilei Tu, Maciej Daniszewski, Camden Lo, Raymond C. Wong, Jonathan G. Crowston, Alice Pébay, Anna E. King, Bang V. Bui, Guei-Sheung Liu, Alex W. Hewitt |
| Abstract |
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) has recently been adapted to enable efficient editing of the mammalian genome, opening novel avenues for therapeutic intervention of inherited diseases. In seeking to disrupt yellow fluorescent protein (YFP) in a Thy1-YFP transgenic mouse, we assessed the feasibility of utilizing the adeno-associated virus 2 (AAV2) to deliver CRISPR/Cas for gene modification of retinal cells in vivo. Single guide RNA (sgRNA) plasmids were designed to target YFP, and after in vitro validation, selected guides were cloned into a dual AAV system. One AAV2 construct was used to deliver Streptococcus pyogenes Cas9 (SpCas9), and the other delivered sgRNA against YFP or LacZ (control) in the presence of mCherry. Five weeks after intravitreal injection, retinal function was determined using electroretinography, and CRISPR/Cas-mediated gene modifications were quantified in retinal flat mounts. Adeno-associated virus 2-mediated in vivo delivery of SpCas9 with sgRNA targeting YFP significantly reduced the number of YFP fluorescent cells of the inner retina of our transgenic mouse model. Overall, we found an 84.0% (95% confidence interval [CI]: 81.8-86.9) reduction of YFP-positive cells in YFP-sgRNA-infected retinal cells compared to eyes treated with LacZ-sgRNA. Electroretinography profiling found no significant alteration in retinal function following AAV2-mediated delivery of CRISPR/Cas components compared to contralateral untreated eyes. Thy1-YFP transgenic mice were used as a rapid quantifiable means to assess the efficacy of CRISPR/Cas-based retinal gene modification in vivo. We demonstrate that genomic modification of cells in the adult retina can be readily achieved by viral-mediated delivery of CRISPR/Cas. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 38% |
| Germany | 1 | 13% |
| Australia | 1 | 13% |
| Unknown | 3 | 38% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 88% |
| Scientists | 1 | 13% |
Mendeley readers
The data shown below were compiled from readership statistics for 197 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | <1% |
| Unknown | 196 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 42 | 21% |
| Student > Ph. D. Student | 41 | 21% |
| Student > Bachelor | 34 | 17% |
| Student > Master | 19 | 10% |
| Student > Doctoral Student | 8 | 4% |
| Other | 18 | 9% |
| Unknown | 35 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 54 | 27% |
| Agricultural and Biological Sciences | 38 | 19% |
| Neuroscience | 26 | 13% |
| Medicine and Dentistry | 15 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 7 | 4% |
| Other | 16 | 8% |
| Unknown | 41 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 44. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 January 2024.
All research outputs
#943,372
of 25,374,647 outputs
Outputs from Investigative Ophthalmology & Visual Science
#108
of 7,983 outputs
Outputs of similar age
#17,943
of 367,290 outputs
Outputs of similar age from Investigative Ophthalmology & Visual Science
#5
of 156 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 7,983 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 367,290 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 156 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.