| Title |
Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)
|
|---|---|
| Published in |
VirusDisease, May 2016
|
| DOI | 10.1007/s13337-016-0321-0 |
| Pubmed ID | |
| Authors |
Gholamhosein Pourghanbari, Hasan Nili, Afagh Moattari, Ali Mohammadi, Aida Iraji |
| Abstract |
Lemon balm derivatives are going to acquire a novelty as natural and potent remedy for treatment of viral infections since the influenza viruses are developing resistance to the current antivirals widely. Oseltamivir, Melissa officinalis essential oil (MOEO) and their synergistic efficacy against avian influenza virus (AIV) subtype H9N2 were evaluated in vitro in MDCK cells at different time exposure by using TCID50, HA, Real Time PCR and HI assay. The results showed that MOEO could inhibit replication of AVI through the different virus replication phase (P ≤ 0.05). Also the highest antiviral activity of MOEO was seen when AIV incubated with MOEO before cell infection. The TCID50/ml was reduced 1.3-2.1, 2.3-2.8, 3.7-4.5 log 10 than control group (5.6 log 10), HAU/50 µl was decreased 85-94, 71.4-94, 71.4-94 % and viral genome copy number/µl was brought down 68-95, 90-100, 89.6-99.9 % at pre-infection, post-infection and simultaneous stage, respectively. Hemagglutination inhibition result showed the MOEO was not able to inhibit agglutination of the chicken red blood cell (cRBC). Replication of the AVI was suppressed by the different concentration of oseltamivir completely or near 100 %. Also oseltamivir showed a synergistic activity with MOEO especially when oseltamivir concentration reduced under 0.005 mg/ml. The chemical composition was examined by GC-MS analysis and Its main constituents were identified as monoterpenaldehydes citral a, citral b. In conclusion, the findings of the study showed that lemon balm essential oil could inhibit influenza virus replication through different replication cycle steps especially throughout the direct interaction with the virus particles. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 29% |
| India | 1 | 14% |
| Spain | 1 | 14% |
| Unknown | 3 | 43% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 43% |
| Practitioners (doctors, other healthcare professionals) | 3 | 43% |
| Science communicators (journalists, bloggers, editors) | 1 | 14% |
Mendeley readers
The data shown below were compiled from readership statistics for 128 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 128 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 17 | 13% |
| Researcher | 16 | 13% |
| Student > Ph. D. Student | 12 | 9% |
| Student > Master | 6 | 5% |
| Other | 5 | 4% |
| Other | 27 | 21% |
| Unknown | 45 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 14 | 11% |
| Biochemistry, Genetics and Molecular Biology | 13 | 10% |
| Chemistry | 9 | 7% |
| Medicine and Dentistry | 7 | 5% |
| Other | 20 | 16% |
| Unknown | 50 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 54. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 December 2023.
All research outputs
#764,535
of 24,937,289 outputs
Outputs from VirusDisease
#5
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Outputs of similar age
#14,567
of 340,711 outputs
Outputs of similar age from VirusDisease
#1
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Altmetric has tracked 24,937,289 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 368 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done particularly well, scoring higher than 98% of its peers.
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