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Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry.

Overview of attention for article published in Cold Spring Harbor Protocols, July 2016
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1 X user

Citations

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439 Mendeley
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Title
Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry.
Published in
Cold Spring Harbor Protocols, July 2016
DOI 10.1101/pdb.prot087163
Pubmed ID
Authors

Lisa C Crowley, Adrian P Scott, Brooke J Marfell, Jeanne A Boughaba, Grace Chojnowski, Nigel J Waterhouse

Abstract

Propidium iodide (PI) is a small fluorescent molecule that binds to DNA but cannot passively traverse into cells that possess an intact plasma membrane. PI uptake versus exclusion can be used to discriminate dead cells, in which plasma membranes become permeable regardless of the mechanism of death, from live cells with intact membranes. PI is excited by wavelengths between 400 and 600 nm and emits light between 600 and 700 nm, and is therefore compatible with lasers and photodetectors commonly available in flow cytometers. This protocol for PI staining can be used to quantitate cell death in most modern research facilities and universities.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 439 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Poland 1 <1%
Unknown 438 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 81 18%
Student > Ph. D. Student 75 17%
Student > Master 60 14%
Researcher 34 8%
Student > Doctoral Student 19 4%
Other 27 6%
Unknown 143 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 119 27%
Agricultural and Biological Sciences 36 8%
Pharmacology, Toxicology and Pharmaceutical Science 26 6%
Medicine and Dentistry 24 5%
Immunology and Microbiology 22 5%
Other 56 13%
Unknown 156 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 July 2016.
All research outputs
#18,465,704
of 22,880,230 outputs
Outputs from Cold Spring Harbor Protocols
#622
of 973 outputs
Outputs of similar age
#267,504
of 351,902 outputs
Outputs of similar age from Cold Spring Harbor Protocols
#16
of 33 outputs
Altmetric has tracked 22,880,230 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 973 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 351,902 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.