Title |
Triggering Apoptosis in Hematopoietic Cells with Cytotoxic Drugs.
|
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Published in |
Cold Spring Harbor Protocols, July 2016
|
DOI | 10.1101/pdb.prot087130 |
Pubmed ID | |
Authors |
Lisa C Crowley, Brooke J Marfell, Adrian P Scott, Nigel J Waterhouse |
Abstract |
Cytotoxic agents are commonly added to cultured cells in the laboratory to investigate their efficacy, mechanism of action, and therapeutic potential. Most of these agents trigger cell death by apoptosis, which is also the most common form of cell death during development, aging, homeostasis, and eradication of disease. Treatment of cells with cytotoxic agents is therefore useful for investigating basic mechanisms of cell death in the human body. Actinomycin D, a cytotoxic agent isolated from Streptomyces, induces apoptosis in a variety of cell lines including the histiocytic lymphoma cell line U937. Treatment of U937 cells with actinomycin D provides an ideal model of drug-induced apoptosis that can also be used as a positive control for comparison with other treatments. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Hungary | 1 | 6% |
Unknown | 17 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 17% |
Student > Master | 2 | 11% |
Researcher | 2 | 11% |
Student > Doctoral Student | 1 | 6% |
Professor | 1 | 6% |
Other | 3 | 17% |
Unknown | 6 | 33% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 5 | 28% |
Agricultural and Biological Sciences | 3 | 17% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 11% |
Immunology and Microbiology | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 33% |