| Title |
Hydrodynamic chromatography coupled to single-particle ICP-MS for the simultaneous characterization of AgNPs and determination of dissolved Ag in plasma and blood of burn patients
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, September 2015
|
| DOI | 10.1007/s00216-015-9014-6 |
| Pubmed ID | |
| Authors |
Marco Roman, Chiara Rigo, Hiram Castillo-Michel, Ivan Munivrana, Vincenzo Vindigni, Ivan Mičetić, Federico Benetti, Laura Manodori, Warren R. L. Cairns |
| Abstract |
Silver nanoparticles (AgNPs) are increasingly used in medical devices as innovative antibacterial agents, but no data are currently available on their chemical transformations and fate in vivo in the human body, particularly on their potential to reach the circulatory system. To study the processes involving AgNPs in human plasma and blood, we developed an analytical method based on hydrodynamic chromatography (HDC) coupled to inductively coupled plasma mass spectrometry (ICP-MS) in single-particle detection mode. An innovative algorithm was implemented to deconvolute the signals of dissolved Ag and AgNPs and to extrapolate a multiparametric characterization of the particles in the same chromatogram. From a single injection, the method provides the concentration of dissolved Ag and the distribution of AgNPs in terms of hydrodynamic diameter, mass-derived diameter, number and mass concentration. This analytical approach is robust and suitable to study quantitatively the dynamics and kinetics of AgNPs in complex biological fluids, including processes such as agglomeration, dissolution and formation of protein coronas. The method was applied to study the transformations of AgNP standards and an AgNP-coated dressing in human plasma, supported by micro X-ray fluorescence (μXRF) and micro X-ray absorption near-edge spectroscopy (μXANES) speciation analysis and imaging, and to investigate, for the first time, the possible presence of AgNPs in the blood of three burn patients treated with the same dressing. Together with our previous studies, the results strongly support the hypothesis that the systemic mobilization of the metal after topical administration of AgNPs is driven by their dissolution in situ. Graphical Abstract Simplified scheme of the combined analytical approach adopted for studying the chemical dynamics of AgNPs in human plasma/blood. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Italy | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 77 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 21% |
| Researcher | 12 | 16% |
| Student > Master | 9 | 12% |
| Student > Bachelor | 8 | 10% |
| Professor | 6 | 8% |
| Other | 16 | 21% |
| Unknown | 10 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 30 | 39% |
| Environmental Science | 8 | 10% |
| Medicine and Dentistry | 7 | 9% |
| Agricultural and Biological Sciences | 4 | 5% |
| Engineering | 4 | 5% |
| Other | 10 | 13% |
| Unknown | 14 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2016.
All research outputs
#15,528,009
of 25,394,764 outputs
Outputs from Analytical & Bioanalytical Chemistry
#4,707
of 9,624 outputs
Outputs of similar age
#144,194
of 286,073 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#54
of 171 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,624 research outputs from this source. They receive a mean Attention Score of 3.1. This one has gotten more attention than average, scoring higher than 50% of its peers.
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We're also able to compare this research output to 171 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.