| Title |
Interleukin-8 reduces post-surgical lymphedema formation by promoting lymphatic vessel regeneration
|
|---|---|
| Published in |
Angiogenesis, September 2012
|
| DOI | 10.1007/s10456-012-9297-6 |
| Pubmed ID | |
| Authors |
Inho Choi, Yong Suk Lee, Hee Kyoung Chung, Dongwon Choi, Tatiana Ecoiffier, Ha Neul Lee, Kyu Eui Kim, Sunju Lee, Eun Kyung Park, Yong Sun Maeng, Nam Yun Kim, Robert D. Ladner, Nicos A. Petasis, Chester J. Koh, Lu Chen, Heinz-Josef Lenz, Young-Kwon Hong |
| Abstract |
Lymphedema is mainly caused by lymphatic obstruction and manifested as tissue swelling, often in the arms and legs. Lymphedema is one of the most common post-surgical complications in breast cancer patients and presents a painful and disfiguring chronic illness that has few treatment options. Here, we evaluated the therapeutic potential of interleukin (IL)-8 in lymphatic regeneration independent of its pro-inflammatory activity. We found that IL-8 promoted proliferation, tube formation, and migration of lymphatic endothelial cells (LECs) without activating the VEGF signaling. Additionally, IL-8 suppressed the major cell cycle inhibitor CDKN1C/p57(KIP2) by downregulating its positive regulator PROX1, which is known as the master regulator of LEC-differentiation. Animal-based studies such as matrigel plug and cornea micropocket assays demonstrated potent efficacy of IL-8 in activating lymphangiogenesis in vivo. Moreover, we have generated a novel transgenic mouse model (K14-hIL8) that expresses human IL-8 in the skin and then crossed with lymphatic-specific fluorescent (Prox1-GFP) mouse. The resulting double transgenic mice showed that a stable expression of IL-8 could promote embryonic lymphangiogenesis. Moreover, an immunodeficient IL-8-expressing mouse line that was established by crossing K14-hIL8 mice with athymic nude mice displayed an enhanced tumor-associated lymphangiogenesis. Finally, when experimental lymphedema was introduced, K14-hIL8 mice showed an improved amelioration of lymphedema with an increased lymphatic regeneration. Together, we report that IL-8 can activate lymphangiogenesis in vitro and in vivo with a therapeutic efficacy in post-surgical lymphedema. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 60 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 15% |
| Researcher | 7 | 12% |
| Student > Master | 7 | 12% |
| Student > Bachelor | 7 | 12% |
| Student > Doctoral Student | 5 | 8% |
| Other | 13 | 22% |
| Unknown | 12 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 21 | 35% |
| Agricultural and Biological Sciences | 7 | 12% |
| Biochemistry, Genetics and Molecular Biology | 6 | 10% |
| Engineering | 5 | 8% |
| Chemistry | 3 | 5% |
| Other | 5 | 8% |
| Unknown | 13 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 November 2013.
All research outputs
#2,322,500
of 22,678,224 outputs
Outputs from Angiogenesis
#32
of 536 outputs
Outputs of similar age
#16,133
of 169,085 outputs
Outputs of similar age from Angiogenesis
#2
of 17 outputs
Altmetric has tracked 22,678,224 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 536 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one has done particularly well, scoring higher than 93% of its peers.
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We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.