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The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation

Overview of attention for article published in Journal of The Royal Society Interface, July 2012
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Title
The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation
Published in
Journal of The Royal Society Interface, July 2012
DOI 10.1098/rsif.2012.0428
Pubmed ID
Authors

Enda P. Dowling, William Ronan, Gidon Ofek, Vikram S. Deshpande, Robert M. McMeeking, Kyriacos A. Athanasiou, J. Patrick McGarry

Abstract

The biomechanisms that govern the response of chondrocytes to mechanical stimuli are poorly understood. In this study, a series of in vitro tests are performed, in which single chondrocytes are subjected to shear deformation by a horizontally moving probe. Dramatically different probe force-indentation curves are obtained for untreated cells and for cells in which the actin cytoskeleton has been disrupted. Untreated cells exhibit a rapid increase in force upon probe contact followed by yielding behaviour. Cells in which the contractile actin cytoskeleton was removed exhibit a linear force-indentation response. In order to investigate the mechanisms underlying this behaviour, a three-dimensional active modelling framework incorporating stress fibre (SF) remodelling and contractility is used to simulate the in vitro tests. Simulations reveal that the characteristic force-indentation curve observed for untreated chondrocytes occurs as a result of two factors: (i) yielding of SFs due to stretching of the cytoplasm near the probe and (ii) dissociation of SFs due to reduced cytoplasm tension at the front of the cell. In contrast, a passive hyperelastic model predicts a linear force-indentation curve similar to that observed for cells in which the actin cytoskeleton has been disrupted. This combined modelling-experimental study offers a novel insight into the role of the active contractility and remodelling of the actin cytoskeleton in the response of chondrocytes to mechanical loading.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 97 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 3%
United Kingdom 2 2%
Ireland 2 2%
Unknown 90 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 30 31%
Student > Master 15 15%
Researcher 14 14%
Student > Doctoral Student 7 7%
Student > Bachelor 7 7%
Other 13 13%
Unknown 11 11%
Readers by discipline Count As %
Engineering 51 53%
Agricultural and Biological Sciences 9 9%
Physics and Astronomy 7 7%
Mathematics 6 6%
Computer Science 2 2%
Other 8 8%
Unknown 14 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 September 2012.
All research outputs
#20,166,700
of 22,678,224 outputs
Outputs from Journal of The Royal Society Interface
#2,836
of 3,044 outputs
Outputs of similar age
#147,006
of 163,891 outputs
Outputs of similar age from Journal of The Royal Society Interface
#35
of 37 outputs
Altmetric has tracked 22,678,224 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,044 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 163,891 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 37 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.