| Title |
Qualitative screening of phenolic compounds in olive leaf extracts by hyphenated liquid chromatography and preliminary evaluation of cytotoxic activity against human breast cancer cells
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, March 2010
|
| DOI | 10.1007/s00216-010-3604-0 |
| Pubmed ID | |
| Authors |
Shaoping Fu, David Arráez-Roman, Antonio Segura-Carretero, Javier A. Menéndez, María P. Menéndez-Gutiérrez, Vicente Micol, Alberto Fernández-Gutiérrez |
| Abstract |
In this work, high-performance liquid chromatography (HPLC) coupled to electrospray time-of-flight mass spectrometry (ESI-TOF-MS) and electrospray ion trap multiple-stage tandem mass spectrometry (ESI-IT-MS(2)) has been applied to screen phenolic compounds in olive leaf extracts. The use of a small particle size C18 column (1.8 micro) provided great resolution and made separation of a lot of isomers possible. The structural characterization was based on accurate mass data obtained by ESI-TOF-MS, and the nature of fragmentation ions were further confirmed by ESI-IT-MS(2) when possible. In addition, we employed tetrazolium salt (MTT)-based assays to assess the effects of olive leaf extracts on the growth of human tumor-derived cells. Upon this approach, we achieved an accurate profile of olive leaf phenolics along with the identification of several important isomers of secoiridoids and flavonoids. This will allow a better understanding of the complete composition of olive-leaf-bioactive compounds as well as their involvement in Olea europaea L. biochemical pathways. Importantly, olive leaf extracts exhibited dose-dependent inhibitory effects on the metabolic status (cell viability) of three breast cancer models in vitro. Since the tumoricidal activity of the extracts should be mainly attributed to the identified olive leaf phenolics, these findings warrant further investigation at the structure-function molecular level to definitely establish the anticancer value of these phytochemicals. |
Mendeley readers
The data shown below were compiled from readership statistics for 131 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | <1% |
| Algeria | 1 | <1% |
| Poland | 1 | <1% |
| Australia | 1 | <1% |
| Unknown | 127 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 31 | 24% |
| Researcher | 18 | 14% |
| Student > Master | 12 | 9% |
| Student > Postgraduate | 8 | 6% |
| Student > Bachelor | 7 | 5% |
| Other | 24 | 18% |
| Unknown | 31 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 29 | 22% |
| Chemistry | 23 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 10 | 8% |
| Medicine and Dentistry | 10 | 8% |
| Biochemistry, Genetics and Molecular Biology | 6 | 5% |
| Other | 19 | 15% |
| Unknown | 34 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 April 2022.
All research outputs
#5,344,962
of 25,394,764 outputs
Outputs from Analytical & Bioanalytical Chemistry
#830
of 9,624 outputs
Outputs of similar age
#22,140
of 103,092 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#15
of 61 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,624 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 103,092 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 61 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.