Title |
CRISPR: a versatile tool for both forward and reverse genetics research
|
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Published in |
Human Genetics, July 2016
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DOI | 10.1007/s00439-016-1704-4 |
Pubmed ID | |
Authors |
Channabasavaiah B. Gurumurthy, M’hamed Grati, Masato Ohtsuka, Samantha L. P. Schilit, Rolen M. Quadros, Xue Zhong Liu |
Abstract |
Human genetics research employs the two opposing approaches of forward and reverse genetics. While forward genetics identifies and links a mutation to an observed disease etiology, reverse genetics induces mutations in model organisms to study their role in disease. In most cases, causality for mutations identified by forward genetics is confirmed by reverse genetics through the development of genetically engineered animal models and an assessment of whether the model can recapitulate the disease. While many technological advances have helped improve these approaches, some gaps still remain. CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated), which has emerged as a revolutionary genetic engineering tool, holds great promise for closing such gaps. By combining the benefits of forward and reverse genetics, it has dramatically expedited human genetics research. We provide a perspective on the power of CRISPR-based forward and reverse genetics tools in human genetics and discuss its applications using some disease examples. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 40% |
Colombia | 1 | 20% |
Unknown | 2 | 40% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 60% |
Scientists | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 145 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 22 | 15% |
Researcher | 17 | 12% |
Student > Ph. D. Student | 16 | 11% |
Student > Master | 16 | 11% |
Unspecified | 7 | 5% |
Other | 22 | 15% |
Unknown | 45 | 31% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 48 | 33% |
Agricultural and Biological Sciences | 21 | 14% |
Unspecified | 7 | 5% |
Medicine and Dentistry | 5 | 3% |
Neuroscience | 3 | 2% |
Other | 16 | 11% |
Unknown | 45 | 31% |