| Title |
Scale-up of human embryonic stem cell culture using a hollow fibre bioreactor
|
|---|---|
| Published in |
Biotechnology Techniques, September 2012
|
| DOI | 10.1007/s10529-012-1033-1 |
| Pubmed ID | |
| Authors |
Iwan Roberts, Stefano Baila, R. Brent Rice, Michiel Etienne Janssens, Kim Nguyen, Nathalie Moens, Ludmila Ruban, Diana Hernandez, Pete Coffey, Chris Mason |
| Abstract |
The commercialisation of human embryonic stem cell derived cell therapies for large patient populations is reliant on both minimising expensive and variable manual-handling methods whilst realising economies of scale. The Quantum Cell Expansion System, a hollow fibre bioreactor (Terumo BCT), was used in a pilot study to expand 60 million human embryonic stem cells to 708 million cells. Further improvements can be expected with optimisation of media flow rates throughout the run to better control the cellular microenvironment. High levels of pluripotency marker expression were maintained on the bioreactor, with 97.7 % of cells expressing SSEA-4 when harvested. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 104 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Portugal | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 101 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 26 | 25% |
| Researcher | 17 | 16% |
| Student > Bachelor | 12 | 12% |
| Student > Master | 11 | 11% |
| Student > Doctoral Student | 5 | 5% |
| Other | 12 | 12% |
| Unknown | 21 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 16% |
| Agricultural and Biological Sciences | 17 | 16% |
| Chemical Engineering | 15 | 14% |
| Engineering | 12 | 12% |
| Medicine and Dentistry | 8 | 8% |
| Other | 8 | 8% |
| Unknown | 27 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 November 2016.
All research outputs
#6,942,318
of 25,411,814 outputs
Outputs from Biotechnology Techniques
#738
of 2,762 outputs
Outputs of similar age
#50,189
of 187,290 outputs
Outputs of similar age from Biotechnology Techniques
#3
of 12 outputs
Altmetric has tracked 25,411,814 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.9. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 187,290 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.