| Title |
The maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer
|
|---|---|
| Published in |
Journal of Molecular Medicine, September 2012
|
| DOI | 10.1007/s00109-012-0949-1 |
| Pubmed ID | |
| Authors |
Ruprecht Kuner, Maria Fälth, Nicole Chui Pressinotti, Jan C. Brase, Sabrina Balaguer Puig, Jennifer Metzger, Stephan Gade, Georg Schäfer, Georg Bartsch, Eberhard Steiner, Helmut Klocker, Holger Sültmann |
| Abstract |
Loss of cell cycle control is a prerequisite for cancer onset and progression. In prostate cancer, increased activity of cell cycle genes has been associated with prognostic parameters such as biochemical relapse and survival. The identification of novel oncogenic and druggable targets in patient subgroups with poor prognosis may help to develop targeted therapy approaches. We analyzed prostate cancer and corresponding benign tissues (n = 98) using microarrays. The comparison of high- and low-grade tumors (Gleason score ≥ 4 + 3 vs. ≤ 3 + 4) revealed 144 differentially expressed genes (p < 0.05). Out of these, 15 genes were involved in the cell cycle process. The gene maternal embryonic leucine zipper kinase (MELK) was identified to be highly correlated with cell cycle genes like UBE2C, TOP2A, CCNB2, and AURKB. Increased MELK gene expression in high-risk prostate cancer was validated by qPCR in an independent patient cohort (p < 0.005, n = 79). Immunohistochemistry analysis using a tissue microarray (n = 94) revealed increased MELK protein expression in prostate cancer tissues of high Gleason scores. RNAi-based inhibition of MELK in PC3 and LNCaP cells suggested putative function in chromatin modification, embryonic development and cell migration. The concerted inhibition of MELK and other cell cycle targets by the antibiotic siomycin A strongly impaired cell viability of prostate cancer cells, and may point to a novel therapy approach for a subset of high-risk prostate cancer patients. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 69 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 26% |
| Student > Ph. D. Student | 17 | 25% |
| Student > Master | 9 | 13% |
| Student > Bachelor | 4 | 6% |
| Student > Doctoral Student | 4 | 6% |
| Other | 10 | 14% |
| Unknown | 7 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 30% |
| Biochemistry, Genetics and Molecular Biology | 19 | 28% |
| Medicine and Dentistry | 7 | 10% |
| Chemistry | 4 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
| Other | 4 | 6% |
| Unknown | 11 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2022.
All research outputs
#3,172,358
of 23,106,934 outputs
Outputs from Journal of Molecular Medicine
#114
of 1,557 outputs
Outputs of similar age
#22,337
of 169,850 outputs
Outputs of similar age from Journal of Molecular Medicine
#1
of 9 outputs
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