New Developments and New Applications in Animal Cell Technology

Table of Contents

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   Book Overview
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   Chapter 1 Genetic Manipulation of the Mammalian Genome
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   Chapter 2 Engineering Glycosylation in Animal Cells
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   Chapter 3 Sessions on: Biosynthesis and Post-Translational Modifications of Recombinant Proteins, and Cell Physiology and Metabolic Engineering of Animal Cells
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   Chapter 4 Enhanced Recombinant Protein Expression in Insect Cells in the Presence of Dimethylsulfoxide (DMSO)
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   Chapter 5 Studies of the Hepatitis C Virus Recombinant Protein Production and Its Effects on SF-9 Insect Cells Metabolism
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   Chapter 6 The Human Multidrug Transporter (MDR1) Expressed in the Baculovirus-SF9 Insect Cell System
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   Chapter 7 Glutamine Synthetase Transfected Cells May Avoid Selection by Releasing Glutamine
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   Chapter 8 Study of Different Cell Culture Conditions for the Production of a “Reshaped” Mab in NSO Cells
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    Chapter 9 Increasing Monoclonal Antibody Productivity by Semicontinuous Substitution of Production Medium for Growth Medium
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    Chapter 10 Expression of Recombinant Human Insulin in Chinese Hamster Ovary Cells is Complicated by Intracellular Insulin-Degrading Enzymes
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    Chapter 11 High Yield Expression of Recombinant Plasma Factors: Use of Recombinant Endoprotease Derivatives In Vivo and In Vitro
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    Chapter 12 The Role of Cell Cycle in Determining Levels of Gene Expression in Cho Cells
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    Chapter 13 Heterogeneity within DHFR-Mediated Gene Amplified Population of Cho Cells Producting Chimeric Antibodies
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    Chapter 14 Immunofluorescence Detection of Recombinant Monoclonal Antibody as a Tool in the Characterization of Chinese Hamster Ovary Cell Lines
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    Chapter 15 Electrolyzed Reduced Water Which Can Scavenge Active Oxygen Species Supresses Cell Growth and Regulates Gene Expression of Animal Cells
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    Chapter 16 Differential Gene Expression of Cytochrome P450 in Immortalised Hepatocyte Cell Lines
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    Chapter 17 Post-Transcriptional Control of Recombinant Gene Expression: mRNA Retargeting and Regulation of Expression
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    Chapter 18 Transient Transfection in Mammalian Cells
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    Chapter 19 Large Scale Transient Gene Expression in Mammalian Cells
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    Chapter 20 Synergistic Enhancement of Transient Expression by Dioleoyl-Melittin (DOM) and Polyethylenimine (PEI) in Mammalian Cells in Suspensionculture
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    Chapter 21 Transient Expression of a Soluble and Secreted form of Heterodimeric T-Cell Receptor in Hek-293
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    Chapter 22 Measurable Parameters of Cells and Precipitate Predict Transfectability with Calcium Phosphate
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    Chapter 23 Influence of Na-Butyrate on the Production and Sialylation of Human Interferon-γ by α2,6-Sialyltransferase Engineered CHO-Cells
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    Chapter 24 Elevated pCO2 Inhibits the Polysialylation of the Neural Cell Adhesion Molecule in CHO MT2-1-8 Cell Cultures
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    Chapter 25 Influence of Cultivation Conditions on Glycosylation Pattern-a Fed-Batch and Continuous Culture Study
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    Chapter 26 Effects of Different Production Systems on Glycosylation Pattern of Murine Monoclonal IgA
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    Chapter 27 Identification of Altered Glycosylation as the Major Difference Between Intracellulary Accumulated and Secreted β-Trace Protein Produced in Baculovirus-Infected Insect Cells
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    Chapter 28 How Ammonium Dominates the Metabolism of in Vitro Cultivated Mammalian Cells
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    Chapter 29 Study of Human Recombinant GM-CSF Produced in Different Host Systems Using Monoclonal Antibodies
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    Chapter 30 Modification of Hybridoma Cells Metabolism
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    Chapter 31 Cloning and Expression of a Cytosolic Sialidase from CHO Cells in a Glutathione S-Tramsferase (GST)-Encoding Expression Vector
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    Chapter 32 Construction of a Novel CHO Cell Line Coexpressing Human GlycosyItransferases and fusion PSGL-1-Immunoglobulin G
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    Chapter 33 Production of Defined Lycosylation Variants of Secreted Human Glycoprotein Therapeutics by Coexpression with Human Recombinant Glycosyl Transferases in BHK-21 Cells
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    Chapter 34 Antisense RNA for the Elimination of Neugc Rresidues from Recombinant Glycoproteins
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    Chapter 35 Intracellular Fatty Acid Composition Affects Cell Yield, Energy Metabolism and Cell Damage in Agitated Cultures
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    Chapter 36 Lipid Requirements of a Recombinant Chinese Hamster Ovary Cell Line (CHO)
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    Chapter 37 Sustained Expression in Proliferation Controled BHK-21 Cells
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    Chapter 38 Cell Growth Inhibition by the IRF-1 System
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    Chapter 39 Correlation between BHK Cell Specific Productivity and Metabolism
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    Chapter 40 Effect of Growth Arrest in BHK Metabolism: On Line Monitoring by 13C and 31P NMR Spectroscopy
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    Chapter 41 Cell Survival in CHO Cell Cultures Grown in a Defined Protein Free Medium
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    Chapter 42 Enhanced Apoptosis in Insect Cells Ciltivated in Simulated Microgravity
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    Chapter 43 Modulation of Apoptosis by BCL-2 Expression Following Amino Acid Deprivation and in High Cell Density Perfusion Cultures
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    Chapter 44 Effect of BCL-2 Expression on Hybridoma Cell Growth During Stressful Conditions
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    Chapter 45 Inhibition of c-jun Expression in F-Mel Cells Causes Cell Cycle Arrest and prevention of Apoptosis
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    Chapter 46 Hippocampal Cells in Culture as a Model to Study Neuronal Apoptosis
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    Chapter 47 Development of Carboxy SNARF-1-AM and Annexin V Assays for the Determination of Apoptosis in Heterogeneous Cultures
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    Chapter 48 Session on Integrated Bioprocessing in Animal Cell Technology
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    Chapter 49 Diauxic Cell Behavior Enables Detoxification of CHO Cell Culture Medium During Fed Batch Cultivation
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    Chapter 50 Effects of Polysaccharide Derived from Tea on Growth of Human Cell Lines in Serum Free Culture
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    Chapter 51 Metabolic Demands of Bti-TN-5B1-4 (High FiveTM) Insect Cells During Growth and After Infection with Baculovirus
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    Chapter 52 Effects of Ammonium and Lactate During Continuous Hybridoma Fermentations In A Fluidized-Bed Bioreactor
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    Chapter 53 Serum Concentration and pH Affect the CD13 Receptor Content of HL60 Cells Cultured in Stirred Bioreactors
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    Chapter 54 Four Regulation-Friendly Serum-Free Media For Mammalian and Insect Cells
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    Chapter 55 Proteolytic Activities in the Baculovirus-Insect Cell Expression System
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    Chapter 56 Development of Proteinase Assays for Improved Cho Cell Cultures
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    Chapter 57 Digital Image Analysis: Quantitative Evaluation of Colored Microscopic Images of Animal Cells
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    Chapter 58 The Viable Cell Monitor: A Dielectric spectroscope for Growth and Metabolic Studies of Animal Cells on Macroporous Beads
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    Chapter 59 Measurements of Changes in Cell Size Distribution to Monitor Baculovirus Infection of Insect Cells
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    Chapter 60 Dead Cell Estimation — a Comparison of Different Methods
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    Chapter 61 Fed-Batch Culture Development Based on Biomass Monitoring
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    Chapter 62 On-Line Monitoring of Protein and Susbtrate/Product Concentration in Mammalian Cell Cultivation Process
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    Chapter 63 Process Control and On-Line Feeding Strategies for Fed-Batch and Dialysis Cultures of Hybridoma Cells
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    Chapter 64 Metabolic Network Analysis of a Hybridoma Cell Line Using Mass Balances and C14-Labelled Glucose and Glutamine
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    Chapter 65 Dynamic Medium Optimization by On-Line Heat Flux Measurement and a Stoichiometric Model in Mammalian Cell Culture
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    Chapter 66 Modeling of Glycoprotein Production by Chinese Hamster Ovary Cells for Process Monitoring and Control
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    Chapter 67 The Temperature Effect in Mammalian Cell Culture an Arrhenius Interprettion
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    Chapter 68 Dielectrophoretic Forces Can be Exploited to Increase the Efficiency of Animal Cell Perfusion Cultures
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    Chapter 69 Use of A New Microcarrier with Two-Dimensional Geometry for the Culture of Anchorage-Dependent Cells in Sonoperfused, Continuously Stirred Tank Reactors
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    Chapter 70 Evaluation of Porous Microcarriers in Fluidized Bed Reactor for Protein Production by Heik 293 Cells
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    Chapter 71 Fluidized Bed Technology: Influence of Fluidization Velocity on Nutrient Consumption and Product Expression
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    Chapter 72 High Density and Scale-up Cultivation of Recombinant Cho Cell Line and Hybridomas with Porous Microcarrier Cytopore
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    Chapter 73 Cell-Settler Perfusion System for the Production and Glycosylation of Human Interferon-γ by Clumped Cells
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    Chapter 74 Disposable Bioreactor for Cell Culture Using Wave-Induced Agitation
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    Chapter 75 Use of miniPerm System for an Efficient Production of Mouse Monoclonal Antibodies
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    Chapter 76 Production of Anti-EGF Receptor hMAB in Hollow Fiber Bioreactors for in Vivo Diagnosis
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    Chapter 77 Optimisation of the Production of VLP’s in 2 Lt Stirred Bioreactors
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    Chapter 78 Direct Capture of Monoclonal Antibodies with a New High Capacity STREAMLINE SP XL Ion Exchanger
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    Chapter 79 The Effect of Different Purification Schemes on the Activity of a Monoclonal Antibody
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    Chapter 80 Interfacing of Protein Product Recovery with an Insect Cell Baculovirus Production System
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    Chapter 81 Preparation of Plasmid DNA Using Displacement Chromatography
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    Chapter 82 Novel Methods for Large-Scale Preparation of Nutrient Media and Buffered Salt Solutions
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    Chapter 83 Large Scale Application of the Semliki Forest Virus System: 5-HT3 Receptor Production
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    Chapter 84 From Applied Research to Industrial Application: the Success Story of Monitoring Intracellular Ribonucleotide Pools
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    Chapter 85 New Technology of Production Recombinant Human Erythropoietin for Peroral Administration
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    Chapter 86 Significance of Multiple Testing on Murine Leukemia Virus of Mouse Hybridomas
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    Chapter 87 Murine Retrovirus Detection Using Mus Dunni Cells and S+L− Cells
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    Chapter 88 The Removal of Model Viruses During the Purification of Human Albumin Using Chromatographic Procedures
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    Chapter 89 Inactivation of Hepatitis A Virus During the Production of Nordimmun
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    Chapter 90 Session on: Viral Vector Production for Gene Therapy
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    Chapter 91 Adeno-Associated Viral Vectors: Principles and in Vivo Use
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    Chapter 92 Novel Retroviral Packaging Cell Lines: Improved Vector Production for Efficient Hematopoietic Progenitor Cell Transduction
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    Chapter 93 Scale-Up and Optimisation Issues in Gene Therapy Viral Vector Production
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    Chapter 94 Comparison of Manufacturing Techniques for Adenovirus Production
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    Chapter 95 Safety Considerations in the Development of New Retroviral and Adenoviral Vectors for Gene Therapy
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    Chapter 96 Gene Therapy for Neuromuscular Disorders: Macrophages as Shuttles for Widespread Targeting
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    Chapter 97 Gene Transfer into Dog Myoblasts
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    Chapter 98 Biodistribution Analysis of a Gene Therapy Vector Using the Polymerase Chain Reaction (PCR) Technique
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     Chapter 99 Session on
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     Chapter 100 The Production of Influenza Virus by Immobilised MDCK Cells
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     Chapter 101 Serum-Free Grown MDCK Cells: An Alternative for Influenza Vaccine Virus Production
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     Chapter 102 Evaluation of the New Medium (MDSS2N), Free of Serum and Animal Proteins, for the Production of Biologicals
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     Chapter 103 Production of High Titre Disabled Infectious Single Cycle (Disc) HSV-2 from a Microcarrier Culture
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     Chapter 104 New Form of the Live Measles Vaccine for Oral Administration
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     Chapter 105 Study of Leningrad-16 Vaccine Strain of Measles Virus Reproduction in Cell Cultures Perspective for Biotechnology
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     Chapter 106 Recent Advances with New Vaccine Adjuvants: From Preclinical to Clinical Development
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     Chapter 107 Vaccination with Recombinant Suicidal DNA/RNA
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     Chapter 108 Growth of Goat Endothelial Cells for the Production of A Veterinary Vaccine
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     Chapter 109 Expression in Insect Cells of the Major Parasite Antigen Associated with Human Resistance to Schistosomiasis
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     Chapter 110 Modulation of CD4 Expression on Helper T Lymphocytes and U937 Cells by Ganglioside GM3 and Its Derivatives
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     Chapter 111 Human Serum in Leukocyte Cultures Producing Human Interferon Alpha
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     Chapter 112 The New Type of Immunomodulator
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     Chapter 113 In Vitro Immunization of Human Peripheral Blood Lymphocytes with Cholera Toxin B Subunit
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     Chapter 114 Session on:
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     Chapter 115 Analysis of Cell Growth in A Fixed Bed Bioreactor Using Magnetic Resonance Spectroscopy and Imaging
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     Chapter 116 Expansion of Human Hematopoietic Progenitor Cells in A Fixed Bed Bioreactor
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     Chapter 117 A Novel Assay to Determine and Quantify the Regulation of Cell Motility and Migration Demonstrated on Hematopotetic Cells
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     Chapter 118 Immortalization of Differentiated Hepatocytes
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     Chapter 119 Fixed-Bed Reactors for Animal Cell Cultivation: An Approach to Artificial Organs
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     Chapter 120 High Density Perfusion Culture of Primary Rat Hepatocytes for Potential Use as a Bioartificial Liver Device
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     Chapter 121 Metabolic Competence and Hormonal Regulation of Primary Porcine Hepatocytes in a 3-D Sandwich Configuration
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     Chapter 122 Novel Mini-Bioreactors for Islet Cell Culture
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     Chapter 123 Cultivation of Skin Cells Suitable for Recovery of Burn Wounds
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     Chapter 124 Tissue Therapy for Treatment of Primary Myodystrophies
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     Chapter 125 Possibility of Application of Thyroid Organ Culture for the Treatment of Persistent Hypothyroidism
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     Chapter 126 Human Cells as Therapeutic Agents
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     Chapter 127 Session on
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     Chapter 128 Embryonic Stem Cell Differentiation Models: Cardiovascular, Myogenic and Neurogenic Development In Vitro
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     Chapter 129 Responses of Human Lung Epithelial Cells (A549) to Pathogenic Infection by Mycoplasma Pneumoniae
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     Chapter 130 Polar Lipid Profiling of Mycoplasma Pneumoniae-Infected Human Lung Epithelial Cells
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     Chapter 131 Comparison of Three Dimensional (3-D) Rat Hepatocyte Cultures in Simulated Microgravity Conditions
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     Chapter 132 High Density Culture of the Human Hepatoma Cell Line HepG2: Long-Term Culture for In Vitro Toxicology
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     Chapter 133 Application of Primary Cultures of Rat Fetal Neurons to the Study of Neurotrophic Action of Peptides
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     Chapter 134 Development and Validation of an Image Analysis System for Single Cell Characterization in Cell Monolayers
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     Chapter 135 Development of an Optically Accessible Perfusion Chamber for In Situ Assays and for Long-term Cultivation of Mammalian Cells
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     Chapter 136 Analysis of Mitogenic Activity of Proteins after Separation by Gel Electrophoresis
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     Chapter 137 Workshop on: The Use of Animal Cells Versus the Use of Transgenic Animals for the Production of Recombinant Proteins
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     Chapter 138 Secretion of Fusion Proteins into Milk by Transgenic Mouse Mammary Epithelium
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     Chapter 139 The Production of Proteins in the Milk of Transgenic Livestock: A Comparison of Micro Injection and Nuclear Transfer
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     Chapter 140 Creation of Mice Expressing Human Antibody by Introduction of a Human Chromosome
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     Chapter 141 Transgenic Technology-A Challenge for Mammalian Cell Culture Production Systems