| Title |
l-Ascorbic acid (vitamin C) induces the apoptosis of B16 murine melanoma cells via a caspase-8–independent pathway
|
|---|---|
| Published in |
Cancer Immunology, Immunotherapy, June 2003
|
| DOI | 10.1007/s00262-003-0407-6 |
| Pubmed ID | |
| Authors |
Jae Seung Kang, Daeho Cho, Young-In Kim, Eunsil Hahm, Yoolhee Yang, Daejin Kim, Daeyoung Hur, Hyunjeong Park, Saic Bang, Young Il Hwang, Wang Jae Lee |
| Abstract |
L-ascorbic acid (vitamin C) has been reported to play a role in the treatment and prevention of cancer. However, its specific mechanistic pathways remain obscure. This study was carried out to identify the sodium ascorbate-induced apoptotic pathway in B16F10 murine melanoma cells. Sodium ascorbate was found to induce the apoptosis of B16F10 murine melanoma in a time- and dose-dependent manner, and this was prevented by pretreatment with N-acetyl- L-cysteine (NAC), a well-known antioxidant. In fact, sodium ascorbate-treated B16F10 melanoma cells showed increased intracellular reactive oxygen species generation (ROS) levels. These results indicate that sodium ascorbate induced apoptosis in B16F10 murine melanoma cells by acting as a prooxidant. We examined the involvement of caspase-8 using a specific caspase-8 inhibitor (z-IETD-fmk) on the sodium ascorbate-induced apoptotic pathway. Cell death was found not to be inhibited by z-IETD-fmk treatment, indicating that sodium ascorbate-induced apoptosis is not mediated by caspase-8. In addition, we detected a reduction in the mitochondrial membrane potential during apoptosis and confirmed cytochrome-c release from mitochondria by immunoblotting. Taken together, it appears that the induction of a prooxidant state by sodium ascorbate and a subsequent reduction in mitochondrial membrane potential are involved in the apoptotic pathway of B16F10 murine melanoma cells, and that this occurs in a caspase-8-independent manner. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Hungary | 1 | 2% |
| Portugal | 1 | 2% |
| Brazil | 1 | 2% |
| China | 1 | 2% |
| Lebanon | 1 | 2% |
| Unknown | 38 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 23% |
| Researcher | 6 | 14% |
| Student > Master | 5 | 12% |
| Student > Bachelor | 4 | 9% |
| Professor > Associate Professor | 4 | 9% |
| Other | 2 | 5% |
| Unknown | 12 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 35% |
| Medicine and Dentistry | 6 | 14% |
| Biochemistry, Genetics and Molecular Biology | 4 | 9% |
| Arts and Humanities | 1 | 2% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 15 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 July 2016.
All research outputs
#20,656,161
of 25,374,647 outputs
Outputs from Cancer Immunology, Immunotherapy
#2,569
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Outputs of similar age
#49,838
of 52,474 outputs
Outputs of similar age from Cancer Immunology, Immunotherapy
#8
of 8 outputs
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