| Title |
The role of the C8 proton of ATP in the catalysis of shikimate kinase and adenylate kinase
|
|---|---|
| Published in |
BMC Molecular and Cell Biology, August 2012
|
| DOI | 10.1186/1471-2091-13-15 |
| Pubmed ID | |
| Authors |
Colin P Kenyon, Robyn L Roth |
| Abstract |
It has been demonstrated that the adenyl moiety of ATP plays a direct role in the regulation of ATP binding and/or phosphoryl transfer within a range of kinase and synthetase enzymes. The role of the C8-H of ATP in the binding and/or phosphoryl transfer on the enzyme activity of a number of kinase and synthetase enzymes has been elucidated. The intrinsic catalysis rate mediated by each kinase enzyme is complex, yielding apparent KM values ranging from less than 0.4 μM to more than 1 mM for ATP in the various kinases. Using a combination of ATP deuterated at the C8 position (C8D-ATP) as a molecular probe with site directed mutagenesis (SDM) of conserved amino acid residues in shikimate kinase and adenylate kinase active sites, we have elucidated a mechanism by which the ATP C8-H is induced to be labile in the broader kinase family. We have demonstrated the direct role of the C8-H in the rate of ATP consumption, and the direct role played by conserved Thr residues interacting with the C8-H. The mechanism by which the vast range in KM might be achieved is also suggested by these findings. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| Unknown | 39 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 25% |
| Student > Master | 8 | 20% |
| Researcher | 6 | 15% |
| Student > Doctoral Student | 2 | 5% |
| Other | 2 | 5% |
| Other | 5 | 13% |
| Unknown | 7 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 33% |
| Agricultural and Biological Sciences | 5 | 13% |
| Chemistry | 5 | 13% |
| Medicine and Dentistry | 3 | 8% |
| Immunology and Microbiology | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 10 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2012.
All research outputs
#15,170,530
of 25,374,917 outputs
Outputs from BMC Molecular and Cell Biology
#668
of 1,233 outputs
Outputs of similar age
#109,310
of 185,641 outputs
Outputs of similar age from BMC Molecular and Cell Biology
#8
of 20 outputs
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